Reagents and instruments for immunology, cell biology and molecular biology.
 
> MOUSE IgG3 antibody (ATTO 532)

MOUSE IgG3 antibody (ATTO 532)

Print Print
Please log in

Cat# orb347456-500ug

Size : 500ug

Brand : Biorbyt

Contact local distributor :


Phone : +1 850 650 7790

    • Home
    • MOUSE IgG3 antibody (ATTO 532) [Out of stock]

    MOUSE IgG3 antibody (ATTO 532) [Out of stock]

    Catalog Number: orb347456

    Catalog Numberorb347456
    CategoryAntibodies
    DescriptionMOUSE IgG3 antibody (ATTO 532)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsDOT, FC, FLISA, IF, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG3 heavy chain
    Concentration1.0 mg/mL
    Dilution rangeFLISA: >1:20,000, FC: 1:500 - 1:2,500, IF: >1:5,000, WB: 1:4,000 - 1:20,000
    Form/AppearanceLyophilized
    PurityAnti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
    ConjugationATTO-532
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesgoat anti-MOUSE IgG3 Antibody ATTO532 Conjugation,
    NoteFor research use only
    Application notesAnti-Mouse IgG3 ATTO 532 Antibody has been tested by dot blot. Mouse IgG3 secondary antibody is available in a variety of formats. ATTO dye conjugated antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
    Expiration Date12 months from date of receipt.
    MOUSE IgG3 antibody (ATTO 532) [Out of stock]

    ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.

    MOUSE IgG3 antibody (ATTO 532) [Out of stock]

    Dot Blot of Goat anti-Mouse IgG3 Antibody ATTO 532 Conjugated. Antigen: Mouse IgG3. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: Goat anti-Mouse IgG3 Antibody ATTO 532 Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT.