NEFL ELISA Kit (Human)

Cat# OKEH02111

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02111
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NEFL ELISA Kit (Human) (OKEH02111) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NEFL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NEFL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NEFL captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 9.7% (n = 20)
ELISA Kit Component
ComponentAmount
NEFL Microplate96 Wells (12 x 8 Well strips)
NEFL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated NEFL Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Neurofilament light polypeptide
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNEFL
Gene Full Nameneurofilament light chain
Alias Symbols68 kDa neurofilament protein;CMT1F;CMT2E;CMTDIG;light molecular weight neurofilament protein;neurofilament light polypeptide;neurofilament protein, light chain;neurofilament subunit NF-L;neurofilament triplet L protein;neurofilament, light polypeptide 68kDa;NF68;NFL;NF-L;PPP1R110;protein phosphatase 1, regulatory subunit 110.
NCBI Gene Id4747
Protein NameP07196
Description of TargetNeurofilament light polypeptide
Uniprot IDhttps://www.uniprot.org/uniprot/P07196
Protein Accession #NP_006149.2
Nucleotide Accession #NM_006158.4
Protein Size (# AA)543
Molecular Weight62 kDa

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