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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum (A) and rat cerebellum (B) with NEFL monoclonal antibody, clone NR-4 (Cat # MAB14384).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against native pig NEFL.

    Immunogen

    Crude neurofilament preparation from porcine spinal cord.

    Host

    Mouse

    Theoretical MW (kDa)

    68

    Reactivity

    Human, Pig, Rat

    Form

    Liquid

    Purification

    Protein A/G purification

    Isotype

    IgG1

    Recommend Usage

    Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
    Immunofluorescence (1-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 10 mM PBS.

    Storage Instruction

    Store at -20 to -80°C.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum (A) and rat cerebellum (B) with NEFL monoclonal antibody, clone NR-4 (Cat # MAB14384).

    Immunofluorescence

    Flow Cytometry