Phalloidin, CF®405M
Cat# 00034-T
Size : 50U
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
Phalloidin Conjugates
A highly selective actin filament stain for fixed and permeabilized cells. Labeled phalloidins have similar affinity for both large and small filaments.
Technology
Cellular Stains
CF® Dye & Other Bioconjugates
CF® Dyes
This product is available by special order only.
Please fill in the inquiry form and we will contact you shortly.
Please fill in the inquiry form and we will contact you shortly.
Product Description
A toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. When conjugated to a fluorescent label, it will stain actin filaments in fixed and permeabilized cells.
- Choice of 17 bright & photostable CF® Dyes, biotin, or other common fluorophores
- Dye options for super-resolution and 2-photon imaging
- Excellent signal to background with negligible non-specific staining
Fluorescently-labeled phalloidins bind to F-actin with nanomolar affinity. Labeled phalloidins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and nonmuscle cells from various species of plants, animals and fungi. Different from antibodies, the binding affinity of phalloidin does not change significantly with actin among different species.
Fluorescently-labeled phalloidin is typically used to stain fixed and permeabilized cells. Labeled phalloidins are not cell-permeant and have therefore not been used extensively with living cells, however they can be loaded into live cells via cationic liposomes.
Note: Phalloidin stock solutions are very stable. But binding of fluorescent phalloidins to cells is more labile than other probes, such as antibodies, and staining can become non-specific or lost over time. For best results, store phalloidin-stained samples in a suitable mounting medium at 4°C, protected from light. For certain phalloidin conjugates, especially CF®405M, CF®647, and CF®680, we recommend imaging immediately or shortly after staining. Staining with our other CF® Dye phalloidins usually is stable for up to a week when specimens are stored at 4°C, protected from light.
Superior CF® Dyes
Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.
Super-Resolution Microscopy
CF®647 and CF®680 phalloidins are recommended mainly for STORM applications. Certain CF® Dyes are compatible with various super resolution imaging techniques. The superior brightness, photostability, and photochemical switching properties of certain CF® Dyes are ideal for 3-D SIM, 3-D STORM, and other super-resolution and single-molecule imaging approaches. Learn more about CF® Dyes for super-resolution microscopy.
Phalloidin Conjugates
| Product | Conjugation | Ex/Em | Size | Catalog No. | Purchase |
|---|---|---|---|---|---|
| Biotin-XX Phalloidin | Biotin-XX | N/A | 100 U | 00028 | Purchase 00028 |
| CF®350 Phalloidin | CF®350 | 347/448 nm | 50 U | 00049-T | Purchase 00049-T |
| 300 U | 00049 | Purchase 00049 | |||
| CF®405M Phalloidin | CF®405M | 408/452 nm | 50 U | 00034-T | Purchase 00034-T |
| 300 U | 00034 | Purchase 00034 | |||
| CF®430 Phalloidin | CF®430 | 426/498 nm | 50 U | 00054-T | Purchase 00054-T |
| 300 U | 00054 | Purchase 00054 | |||
| CF®440 Phalloidin | CF®440 | 440/515 nm | 50 U | 00055-T | Purchase 00055-T |
| 300 U | 00055 | Purchase 00055 | |||
| CF®488A Phalloidin | CF®488A | 490/515 nm | 50 U | 00042-T | Purchase 00042-T |
| 300 U | 00042 | Purchase 00042 | |||
| CF®532 Phalloidin | CF®532 | 527/558 nm | 50 U | 00051-T | Purchase 00051-T |
| 300 U | 00051 | Purchase 00051 | |||
| CF®543 Phalloidin | CF®543 | 541/560 nm | 50 U | 00043-T | Purchase 00043-T |
| 300 U | 00043 | Purchase 00043 | |||
| CF®568 Phalloidin | CF®568 | 562/583 nm | 50 U | 00044-T | Purchase 00044-T |
| 300 U | 00044 | Purchase 00044 | |||
| CF®583R Phalloidin | CF®583R | 586/609 nm | 50 U | 00064-T | Purchase 00064-T |
| 300 U | 00064 | Purchase 00064 | |||
| CF®594 Phalloidin | CF®594 | 593/614 nm | 50 U | 00045-T | Purchase 00045-T |
| 300 U | 00045 | Purchase 00045 | |||
| CF®633 Phalloidin | CF®633 | 630/650 nm | 50 U | 00046-T | Purchase 00046-T |
| 300 U | 00046 | Purchase 00046 | |||
| CF®640R Phalloidin | CF®640R | 642/662 nm | 50 U | 00050-T | Purchase 00050-T |
| 300 U | 00050 | Purchase 00050 | |||
| CF®647 Phalloidin* | CF®647 | 650/665 nm | 50 U | 00041-T | Purchase 00041-T* |
| 300 U | 00041 | Purchase 00041* | |||
| CF®660C Phalloidin | CF®660C | 667/685 nm | 50 U | 00052-T | Purchase 00052-T |
| 300 U | 00052 | Purchase 00052 | |||
| CF®660R Phalloidin | CF®660R | 663/682 nm | 50 U | 00047-T | Purchase 00047-T |
| 300 U | 00047 | Purchase 00047 | |||
| CF®680 Phalloidin* | CF®680 | 681/698 nm | 50 U | 00053-T | Purchase 00053-T* |
| 300 U | 00053 | Purchase 00053* | |||
| CF®680R Phalloidin | CF®680R | 680/701 nm | 50 U | 00048-T | Purchase 00048-T |
| 300 U | 00048 | Purchase 00048 | |||
| Fluorescein Phalloidin | Fluorescein | 496/516 nm | 300 U | 00030 | Purchase 00030 |
| Rhodamine Phalloidin | Rhodamine | 540/565 nm | 300 U | 00027 | Purchase 00027 |
| Rhodamine 110 Phalloidin | Rhodamine 110 | 502/524 nm | 300 U | 00032 | Purchase 00032 |
| Sulforhodamine 101 (Texas Red®) Phalloidin | Texas Red® | 591/608 nm | 300 U | 00033 | Purchase 00033 |
Note: CF®555 Phalloidin (00040, 00040-T) has been discontinued. We recommend CF®568 Phalloidin as a replacement for conventional microscopy, or CF®583R Phalloidin as a replacement for STORM.
CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.
References
Download a list of CF® dye Phalloidin references.