sarcomeric alpha Actinin antibody [EA-53]
Cat# GTX29465-500ul
Size : 500ul
Brand : GeneTex
1
Host | Mouse |
---|---|
Clonality | Monoclonal |
Clone Name | EA-53 |
Isotype | IgG1 |
Application | ICC/IF, IHC-P, IHC-Fr, IHC-Wm |
Reactivity | Human, Mouse, Zebrafish |
Package | 500 μl |
View product citations for antibody GTX29465 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application | Recommended Dilution |
---|---|
ICC/IF | Assay dependent |
IHC-P | 1:20‐1:30 |
IHC-Fr | Assay dependent |
IHC-Wm | Assay dependent |
Note : IHC-PRecommendations for antigen retrieval : EDTA buffer pH 8.0 at room temperature for 30 mins. |
Not tested in other applications.
Product Note
This antibody is specific for α-skeletal muscle actinin and α-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Antigen Species
Rabbit
Immunogen
Purified rabbit skeletal α-actinin.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_385929
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actinin alpha 3
Database
DATA IMAGES
GTX29465 IHC-P ImageIHC-P analysis of human skeletal muscle tissue using GTX29465 sarcomeric alpha Actinin antibody [EA-53]. |
GTX29465 IHC-P ImageThe data was published in the journal Cell Death Dis in 2018. PMID: 29371598 |
Application Reference
Nguyen NUN et al. Biochem Biophys Rep 2024; 39 : 101762 Actin-organizing protein palladin modulates C2C12 cell fate determination.
Ma W et al. Nat Commun 2024; 15 (1) : 2137 N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration.
Gong R et al. Pharmacol Res 2021; : 105845 Loss of m6A methyltransferase METTL3 promotes heart regeneration and repair after myocardial injury.
Application : IHC-Fr
Reactivity : Mouse
Bellmann J et al. Biomaterials 2019; 225 : 119537 A customizable microfluidic platform for medium-throughput modeling of neuromuscular circuits.
Application : ICC/IF
Reactivity : Human
Williams AL et al. Physiol Genomics 2018; 50 (7) : 479-494 HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.
Submit a Reference REVIEW
sarcomeric alpha Actinin antibody [EA-53] Cat. No. GTX29465
Rating | | ( Average 5 based on 1 users reviews) |
Immunocytochemistry/ Immunofluorescence(ICC/IF) | | ( Average 5 based on 1 users reviews) |
Application Reference
Nguyen NUN et al. Biochem Biophys Rep 2024; 39:101762 Actin-organizing protein palladin modulates C2C12 cell fate determination.
Ma W et al. Nat Commun 2024; 15 (1):2137 N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration.
Gong R et al. Pharmacol Res 2021;:105845 Loss of m6A methyltransferase METTL3 promotes heart regeneration and repair after myocardial injury.
Application : IHC-Fr
Reactivity : Mouse
Bellmann J et al. Biomaterials 2019; 225:119537 A customizable microfluidic platform for medium-throughput modeling of neuromuscular circuits.
Application : ICC/IF
Reactivity : Human
Williams AL et al. Physiol Genomics 2018; 50 (7):479-494 HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.
Milan M et al. Cell Death Dis 2018; 9 (2):108 Givinostat reduces adverse cardiac remodeling through regulating fibroblasts activation.
Application : ICC/IF
Reactivity : Mouse
Clone Reference
Zou J et al. Elife 2015; 4:e09406 An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Burridge PW et al. PLoS One 2011; 6 (4):e18293 A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability.
Application : ICC/IF
Reactivity : Human
SDS | |
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Sodium Azide.pdf |
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