Canvax Biotech
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Canvax is a leading manufacturer and supplier of the most innovative solutions, kits and R&D Reagents inside Molecular Biology fields. Since its foundation in 2001, Canvax offers reliable, cost-effective and easy-to-use innovative tools for research. Based in Córdoba (Spain), since its beginning it has focused on R&D of multiplex high throughput platforms (HTS) for Drug discovery and Diagnostic applied biosensors. Over a decade later, Canvax is a worldwide leading expert in GPCR expression in heterologous cells, with important patents and exclusive know-how. Canvax prides itself to be the first company to get an unprecedented milestone that could revolutionize the Diagnostics sector: Canvax established stable high-level expression of odour GPCRs into heterologous cell lines in 2014. This key milestone, that could be applied in diagnostic, perfume and cosmetic industry, are being employed to obtain a molecular nose, a sensitive and non-invasive diagnostic molecular device for early detection of cancer, as the nose of trained dogs do. Thanks to this project, Canvax was awarded, in 2013, with the largest Public & Private Contract for Pre competitive Technology Development in Spanish history. Website : www.lifescience.canvaxbiotech.com |
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Classical DNA polymerases for long fragments
The polymerase chain reaction (PCR) has been used extensively in the amplification of DNA fragments where there is a general size preference for products less than 1000 bp. Larger products may be synthesized at low or inefficient levels and then normally after considerable effort in reaction optimization. The maximum amplifiable length of PCR is limited by the low fidelity of the Thermus aquaticus (Taq) DNA polymerase, the most commonly used thermostable polymerase. This limitation is thought to be due to the error‐prone nature of the Taq polymerase, whereby those transcripts that end in an error (a misincorporated base) are not efficiently reinitiated and extended.
Long range PCR allows the amplification of PCR products, which are much larger than those achieved with conventional Taq polymerases. Up to 27 kb fragments are possible from good quality genomic DNA. Amplification of long DNA fragments is desirable for numerous applications such as physical mapping applications and direct cloning from genomes.
Name | Long Taq DNA Polymerase | DNA
polymerase I Large fragment |
LAmp DNA polymerase |
Product code | NB-03-0110 and NB-03-0112 | NB-15-1406 and NB-15-1407 | P301 |
Type | Mix of Taq polymerase and Pfu polymerase | Cloned DNA Polymerase (klenow fragment) gene | Mix of Taq DNA Polymerase and proofreading polymerase |
Speed | - | - | 1 kb/min |
Amplicon size | < 40 kb | - | > 20 kb |
Error rate | 3.6 x10-6 errors / nt | - | 4.1x10-6 errors / nt |
3'-5' exonuclease activity | Yes | Yes | Yes |
HotStart | No | No | No |
Search result : 5 product found
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