Reagents and instruments for immunology, cell biology and molecular biology.
 

Funakoshi

 

Funakoshi Co, Ltd has been involved in the distribution of reagents and research instruments for researchers in the life sciences since its foundation. The company's mission is to provide a wide range of products:

  • Products for molecular biology
  • Antibodies
  • Assay kits
  • Cytokines and growth factors
  • Cell culture products
  • Proteins and enzymes
  • Biochemicals ...

 

Learn more :

 

 

  

 

         

AllView PAGE buffer

 

   

ULTRARIPA Kit for Lipid Raft

 

      

S-Palmitoylation Detection and

Analysis Kit

     

 

         

Long single strand DNA

preparation kit

 

  

Lipid Peroxidation Detection

Tools

 

  

DynaMarker, Prestain Marker

For Small RNA

 

 

 

 
> Funakoshi > Products for research > Immunohistochemistry (IHC) products > Buffers and reagents for IHC > IHC buffers - Quenching > IHC buffers - Quenching - HRP
IHC buffers - Quenching - HRP

IHC buffers - Quenching - HRP


Chromogenic detection methods often use a conjugated enzyme to visualize epitope-antibody interactions. When using this method of detection, the endogenous activity of the same enzyme must be blocked. For example, protocols that include horseradish peroxidase (HRP) or alkaline phosphatase (AP) may require reagents to prevent non-specific signals. Tissues such as kidney, liver, or vascular areas with red blood cells, contain endogenous peroxidase activity. Peroxidase blocking reagents formulated with 3-10% H2O2 can be used to prevent endogenous peroxidase from cleaving the substrate. Endogenous AP found in intestine, kidney, lymphoid and other tissue can be blocked with 1 mM Levamisole. The intestinal form of AP is unaffected by Levamisole but can be blocked by using 1% acetic acid.

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