TransGen Biotech
TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, their products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
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Website : www.transgenbiotech.com
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Library quantifcation master mix for Illumina®
- DNA polymerase
- dNTPs
- MgCl2
- Primers to amplify only the molecules that contain the two sequences of adapters
- SYBR green or EvaGreen
- A ROX solution to add depending on the device used. If the ROX solution is separate from the mix, it is a universal kit that can be used with all instruments. If ROX is contain in the master mix, it is necessary to use the appropriate kits with your device.
Universal | All instruments |
Without ROX | Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler. |
Low ROX | Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P |
High ROX | Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus |
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