Kits for the preparation of Illumina libraries - Targeted sequencing

Kits for the preparation of Illumina libraries - Targeted sequencing


Targeted Sequencing (sometimes called Target Amplicon Sequencing) (TAS) is a new generation DNA sequencing technique that focuses on amplicons and specific genes. Targeted sequencing is a fast and cost-effective way to detect known and novel variants in selected gene sets or genomic regions. Gene sequencing can be carried out using several DNA sequencing methods, depending on the scale. Targeted sequencing requires the prior selection and isolation of genes or regions of interest, usually by PCR amplification or capture methods based on hybridisation.
Targeted Next Generation Sequencing (NGS) is a type of Next Generation Sequencing (NGS) that focuses on specific areas of the genome. The most common methods of targeted NSW sequencing include capture by hybridisation and amplicon sequencing. The main difference between the techniques is the method by which the samples are enriched. Hybridization capture is performed in solution using biotinylated oligos (i.e. baits or probes) to capture complementary sequences from the sample bank. Amplicon sequencing uses PCR primers to amplify the sequences of interest.

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