Cell migration and invasion
Cellular migration is a highly integrated, multi-stage process that plays an important role in the progression of various diseases, including cancer, atherosclerosis and arthritis. There are different types and definitions of cell migration.
Cellular invasion is related to and dependent on cell migration, except that cells do more than migrate. Invasive cells move through the extracellular matrix (ECM) in neighboring tissues in a process that involves extracellular matrix degradation and proteolysis.
We propose tests to study migration and cell invasion in two formats:
- Boyden chamber assays consist of a cell culture insert tucked into the well of the cell culture plate. The cells are seeded in the insert and migrate through the pores of the membrane at the bottom thereof.
- Gap closure assays create a defined area through which cells migrate. Cell migration can be monitored in real time by microscopy.
Boyden chamber
Gap closure
Analysis
Quantitative
Qualitative and quantitative
Detection time
End point
End point and real time
Detection method
Plate reader
Microscope
Cell compatibility
Choose membrane pore size to match cell type
Any
Chemoattractant gradient
Yes
No
Sensitivity
Fair
Good
Adaptability to automation
Poor
Good
Most suitable application
Measure effect of chemoattractant on migration rates
Measure differences in migration rates between treated and untreated cells
