Mounting media

For microscopic analysis, stained tissue sections, cytology gynecology preparations, bacteriological specimens or hematological blood smears are usually mounted with aqueous or non-aqueous mounting media and covered with a coverslip. they need to be stored for a long time.

 

• Help keep a sample in place during imaging
• Prevent the sample from drying out
• Match the refractive index more closely for the lens used
• Prevent photobleaching
• Preserve sample over time for long-term storage

The choice of mounting medium depends on the type of sample, the type of microscopy used for the observation, and the technique used (use of the fluorescent marking for example). There is a wide variety of mounting media, they can be composed of organic solvents such as toluene and xylene or water-based, these media are called aqueous mounting media.