One-Step RT-qPCR mixes - SYBR Green

One-Step RT-qPCR mixes - SYBR Green


As its name implies, single-step RT-PCR combines the synthesis of complementary DNA (cDNA) of the first strand (reverse transcript, RT) and subsequent PCR in a single reaction tube. This reaction configuration simplifies the work, reduces variations and minimizes possible contamination. One-step RT-PCR makes it easier to process a large number of samples, allowing for high-throughput applications. However, one-step RT-PCR uses specific gene primers for amplification, limiting the analysis to a few genes per RNA sample. Since the reaction is a compromise between reverse transcription and amplification conditions, one-step RT-PCR may be less sensitive and less effective in some cases. However, the use of a gene-specific primer in RT-PCR can help maximize the yield of the target cDNA and minimize amplification.


One-step-RT-PCR


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Cat#
Description
Cond.
Price Bef. VAT
AQ211-01
 100rxns×20ulsystem 
AQ311-01
 100rxns×20ulsystem 
AQ311-02
 400rxns×20ulsystem 
AQ211-02
 400rxns×20ulsystem 
orb1733658
 2x1.25ml 
orb1733653
 2x1.25ml 
orb1098416-100rxns
 100reactions(20μlvol) 
orb640199-1000Rxns
 1000Rxns 
orb572669-10x1.25ml
 10x1.25ml 
orb867347-10x1.25ml
 10x1.25ml 
orb1098414-100rxns
 100reactions(20μlvol) 
orb762369-10x1.25ml
 10x1.25ml 
orb1098412-100rxns
 100rxns 
orb1733647
 2x1.25ml 
orb1733660
 2x1.25ml 
PCR-159S
 192reactionsx20µl 
orb1733656
 2x1.25ml 
orb653793-10x1.25ml
 10x1.25ml