SYBR Green Mixes Low ROX for RT-qPCR
SYBR Green Low Rox quantitative RT-PCR mixes are made for use with qPCR devices requiring low ROX as an internal reference dye for fluorescent signal normalization and correction of optical well-to-well variations.
The SYBR Green mix consists of all the necessary components (except template DNA and primers): a Reverse transcriptase, Taq polymerase, dNTPs, MgCl2, SYBR Green and Low ROX dye.
Here's how to select the appropriate ROX dye for the qPCR device you are using:
| Universal | All instruments |
| Without ROX | Bio-Rad
CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2,
Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler. |
| Low ROX | Applied
Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P |
| High ROX | Applied
Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus |
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