Antibody-coupled fluorescent microspheres are microspheres that have been conjugated with antibodies and labeled with a fluorescent dye.

These microspheres can be used in various applications, such as detecting trace amounts of specific antigens in solution and depleting them from solution. They can also be used in immunoassays to detect antibodies against specific antigens.

The process of coupling antigens to fluorescent beads involves confirming that the fluorescent beads can be successfully coupled to antigens. The size of the microspheres can influence specific recognition of antibody-expressing cells, with smaller diameter beads being more likely to be selected.