Cobalt-NTA FPLC columns (Co-NTA)
HiFliQ columns pre-packed and ready to use
with pre-charged super Cobalt-NTA Agarose
resin for affinity purification of poly-histidine tagged
recombinant proteins by immobilized metal ion
affinity chromatography (IMAC).
Available in 1 ml and 5 ml HiFliQ column sizes with
high binding capacity and minimal ion leakage.
Compatible with all common chromatography HPLC
and FPLC instruments (including ÄKTA FPLC's), and
low pressure pumps and syringes using an
appropriate adaptor.
Cobalt NTA resin generally has a lower affinity for the 6x His Tag (proteins elute at a lower Imidazole concentration) but due to its slightly altered binding characteristcs can sometimes provide an increased putiy product when compared to Nickel-NTA resin.
Cobalt NTA resin generally has a lower affinity for the 6x His Tag (proteins elute at a lower Imidazole concentration) but due to its slightly altered binding characteristcs can sometimes provide an increased putiy product when compared to Nickel-NTA resin.
Specifications:
- Column volume: 1 or 5 ml resin
- Column construction: Polypropylene
- Resin: Super Co-NTA Agarose
- Base Matrix: 7.5% cross-linked Agarose
- Co-NTA capacity: 40-50 mg (per 1 ml resin)
- Flow rate: 1 ml/min (1 ml), 1-5 ml/min (5 ml)
- Max pressure: 0.5 MPa (72 psi)
- Universal 10.32 (1/16'') UNF threads:
Inlet Female/Outlet Male
- Dimensions: 15 x 80 mm (1 ml), 23 x 80 mm (5 ml)
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- Chromatography 6
- Concentration/Filtration 4
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