HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
Search result : 4707 product found
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RUOCE / IVD
- Unconjugated 1115
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- FITC 69
- Biotin 68
- PE 55
- APC 50
- MaxLight 490 31
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- MaxLight 405 18
- MaxLight 750 18
- Cy3 15
- Cy5 9
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- Cy7 9
- Alexa Fluor 350 8
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- Alexa Fluor 555 8
- Alexa Fluor 750 8
- PerCp 2
- RBITC 2
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- rabbit 639
- mouse 399
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- 48
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- virus 9
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- bird 5
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- cat 1
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- Primary antibody 1246
- Biochemicals 1225
- Protein/peptide 1000
- ELISA/ELISPOT 367
- Inhibitor/Antagonist/Agonist 324
- Buffers and reagents 158
- RNAi 102
- CRISPR/Cas9 93
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- kit 45
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- Secondary antibody 12
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- Primer 1
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- WB 759
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- IHC 474
- FLISA 121
- ICC 110
- IF 104
- IP 101
- FC 87
- Enzyme-linked immunosorbent assay for Antigen Detection. 10
- 0.078 ng/mL 3
- Chemiluminescence 3
- 0.1 ng/mL 2
- 0.116 ng/ml 1
- ISH 1
- PCR 1
- Polyclonal 661
- 2G6 31
- 4G5 31
- 1F8 30
- 1G1 30
- 1G5 30
- 10i46 25
- 13H261 21
- 3H1166 21
- Monoclonal 21
- M-A261 12
- HLy9.1.25 8
- 1626CT702.263.21 5
- 1A10A10 5
- 10.1F8 4
- 12H6.D3 4
- 13.1G11 4
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- 14F10.H9 4
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- 2E4 4
- 3G8 4
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- 4B9 4
- 5A11 4
- 5A2 4
- 6.1H3.D9.C6 4
- 6.1H8.F9.G6 4
- 6.2D2.E1.D11 4
- 6.7A2.A4.D3 4
- 6.8A2.B8.A11 4
- 6.8E7.A7.A1 4
- 8C7 4
- 8G1 4
- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- Not Applicable 4
- RB21227 4
- 6D23 3
- SAA1697 3
- 12K274 2
- 17D17 2
- 17L18 2
- 202.36 2
- 2213 2
- 2M2 2
- 3F9-2C2 2
- 5B20 2
- 7H111 2
- 8C10 2
- B2M-01 2
- B2M-02 2
- 11C257 1
- 12K99 1
- 134-2C2 1
- 13B822 1
- 5K78 1
- 5K78 (134-2C2) 1
- 6C1 1
- 6C5 1
- 9H177 1
- ADAB-8 1
- DAB-6 1
- Recombinant 1
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