Reagents and instruments for immunology, cell biology and molecular biology.

BRAF probe for ISH CE/IVD - Gastrointestinal pathology

The BRAF gene encodes a protein-serine/threonine kinase that participates in the MAPK cascade, which regulates a large variety of cell processes.

Various BRAF translocations were observed in melanocytic nevi, pilocytic astrocytomas, malignant melanoma, prostate and gastric cancer. The AKAP9-BRAF fusion resulting from paracentric inversion of chromosome 7q was found in radiation-induced papillary thyroid carcinomas.

The fusion proteins contain the protein kinase domain but lack the autoinhibitory N-terminal portion of BRAF resulting in constitutive kinase activity.

In addition, in pilocytic astrocytoma the FAM131B-BRAF fusion has been described resulting from interstitial deletion which removes the BRAF N-terminal inhibitory domain.

Moreover, pancreatic acinar cell carcinoma - a rare subtype of pancreatic cancer with poor prognosis - shows a recurrent SND1-BRAF rearrangement. SND1-BRAF-transformed cells were shown to be sensitive to treatment with a MEK inhibitor.