Our cell migration assays provide a convenient and user-friendly method for measuring 2-dimensional cell migration. When the cells are seeded in the well of the culture plate, they adhere everywhere except at the center of the well where is placed a biocompatible hydrogel point. Once the cells form a monolayer, the test is initiated by gently dissolving the gel with an elimination solution, leaving a gap through which cell migration can occur. The migration of the cells can then be visualized in real time or in end point with a microscope.

 

Unlike traditional Boyden chamber tests, each culture plate can accept cells of any type and size without having to choose a pore size that fits your cell type. For cells that need an extracellular matrix protein for better cell adhesion, we propose 24-well plates pre-coated with collagen I, fibronectin or laminin. We also provide an ECM Array kit that contains a 24-well plate with a row of each of the aforementioned proteins plus a row without a coating to test the best conditions for growing your cells.