MHC Tetramers

The ONLY method to directly measure antigen-specific T cells.

Class I tetramers bind to a distinct set of T cell receptors (TRCs) on a subset of T CD8+ cells, Class II tetramers bind to a distinct population of T CD4+ cells.

The patented MHC iTag tetramers reduce non-specific bindings to allow unique detection and enumeration of Ag specific T cells.

Thus, by mixing Tetramers with PBMCs or whole blood and using flow cytometry as a detection system, a count of all CD4+ or CD8+ T cells that are specific for one peptide and its matched MHC allele is provided, regardless of functionality.

• The only method to detect antigen specific CD4+ or CD8+ T cells ex vivo
• Immune responses are targeted to specific allele
  
• Tetramer staining is quantitative
• Does not require the use of radioactive isotopes
  
• Rapid: allowing fresh blood (or tissue-derived) sample to be analyzed
  
• Large number of samples can be processed using flow cytometer
  
• Tetramer-labeled cells remain viable
  
• All specific CTLs are detected
• Tetramers can be customized for unique applications
  

Specific Mutation reducing non specific binding:

The class 1 MHC present a proprietary mutation of HLA alpha 3 domain region which greatly reduces the non specific binding

Mutated tetramer
Conventional tetramer

 
Diseases:

• Infectious diseases: VIH-SIDA, EBV, CMV, HPV, HBV, HCV, Influenza, Measles, Malaria, TB and RSV
• Cancers: Breast, Prostate, Melanoma, Colon, Lung, Cervical, Ovairian et Leukemia
• Autoimmunes diseases: Diabetes, Lyme disease, Multiple sclerosis, Rheumatoid arthritis, Autoimmun vitiligo
  
• Transplantation: EBV and CMV

Alleles available:

Custom products:

Developed to your:

• allele
• peptide
• fluorochrome (PE or APC)