qPCR for diagnostic
Quantitative PCR (qPCR) also known as real-time quantitative PCR is widely used for diagnostic applications.
QPCR is a technique based on the principle of PCR that is able to simultaneously amplify and detect changes in amplicon concentration. The qPCR collects data during PCR amplification using a fluorescence signal emitted by probes specific for the amplified sequence or DNA binding dyes.
In medical biology, the applications of qPCR are very numerous and cover very diverse fields, the most important of which are in clinical microbiology, oncology and in the study of gene expression. Very important applications have also been developed in food microbiology and food processing.
Clinical microbiology: detection and quantification of bacterial and fungal pathogens; viral titration
Clinical oncology: quantification of fusion transcripts, quantification of oncogenes
Gene expression: estimation of the level of expression of genes of interest, analysis of the expression of gene splicing variants and validation of the expression profiles obtained using DNA arrays
The Monkeypox virus antibody rapid test is a lateral flow chromatographic immunoassay for the qualitative detection of antibodies to Monkeypox Virus in whole blood, serum, or plasma to aid in the diagnosis of Monkeypox Virus infection.
In this test, mouse anti-human IgG and mouse anti-human IgM are coated in the test line regions of the test respectively.
During testing, the antibodies to monkeypox virus in whole blood, serum or plasma...
The Monkeypox virus antigen rapid test is a rapid chromatographic immunoassay intended for the qualitative detection of monkeypox virus A29L antigen in human serum, plasma, whole blood, rash exudate and throat swab specimens.
It is intended for use as an aid in the diagnosis of monkeypox virus infection.
During testing, monkeypox virus antigen reacts with the particle coated with MPX A29L antibody. The mixture migrates upward on the membrane...