ESF AdvanCD Sample Pack

This kit includes 2 L of ESF AdvanCD™ Media, 20 mL of Virus Stabilization Additive (VSA), and 20 mL of Transfection Medium, with each component designed to optimize your research and manufacturing workflows.

ESF AdvanCD™ Media is a high-performance medium that supports the robust growth of suspension Sf9 cell lines, achieving densities exceeding 35 x 10^{^6} cells/mL.

It allows for higher protein expression yields than other media formulations and ensures high reproducibility.

This medium is particularly effective for producing high-titer baculovirus stocks, with titers greater than 5 x 10^{^9} iu/mL, and maintains high baculovirus titers with reduced aggregation when used in conjunction with VSA.

It is scalable for baculovirus infection and protein expression in Spodoptera and Trichoplusia cell lines and adapted for use with Sf9 rhabdovirus negative cell lines, supporting a fully compliant manufacturing platform.

The ready-to-use liquid formulation requires no supplementation, streamlining your workflow.

Transfection Medium is designed to complement ESF 921 and ESF AF insect cell culture media for the cotransfection or transfection steps in baculovirus vector production.

This serum-free, animal-free formulation facilitates vector DNA uptake by insect host cells, leading to higher titers.

It works seamlessly with ESF 921 and ESF AF and is optimized for use with Expres2 for either PEI or lipofection-mediated transfection.

Together, these three products provide a powerful and efficient solution for high-yield baculovirus production and protein expression, ensuring consistency and superior performance in your research and manufacturing processes.