![Flow cytometry](https://www.clinisciences.com/en/upload/thumbs/75-0251-1-1-6wbbnb7q-K4fVj36q-951fe3.png)
Flow cytometry
![Flow cytometry general protocol](https://www.clinisciences.com/en/upload/thumbs/210312_172352_PEEL_rSk5nR-b7ec74.jpg)
I. Required material
Reagents
- A primary antibody , against the target molecule
- A Fc receptor blocker : « Anti-CD16/32, block Fc binding » that non-specifically binds primary antibodies
- A secondary antibody in cases of indirect staining with a non labeled primary antibody
- A non-relevant control isotype with a similar label than primary antibody
Buffers
- A staining buffer
- An intracellular fixation buffer
- Trypan blue...
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![Protocol for flow cytometry on tissues](https://www.clinisciences.com/en/upload/thumbs/180610_171030_PEEL_jQbxMD-d974ce.jpg)
I. Required material
Reagents
- A primary antibody , against the target molecule
- A Fc receptor blocker : « Anti-CD16/32, block Fc binding » that non-specifically binds primary antibodies
- A secondary antibody in cases of indirect staining with a non labeled primary antibody
- A non-relevant control isotype with a similar label than primary antibody
Buffers
- A staining buffer
- An intracellular fixation buffer
- Red Blood...
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![Protocol for flow cytometry on whole blood](https://www.clinisciences.com/en/upload/thumbs/180610_171641_PEEL_9HKRPp-16e66d.jpg)
I. Required material
Reagents
- A primary antibody , against the target molecule
- A secondary antibody in cases of indirect staining with a non labeled primary antibody
- A non-relevant control isotype with a similar label than primary antibody
Buffers
- A staining buffer
- A Red Blood Cells Lysis (RBC) buffer
- An intracellular fixation buffer
- Trypan blue for counting cells
- Gaines sheath fluid
Material
- Pipets and Pipet-aid
-...
![]() |
![Protocol for intracellular flow cytometry](https://www.clinisciences.com/en/upload/thumbs/180610_171329_PEEL_edhSKr-65be2a.jpg)
I. Required material
Reagents
- A primary antibody against the target molecule
- A secondary antibody in cases of indirect staining with a non labeled primary antibody
- A non-relevant control isotype with a similar label than primary antibody
Buffers
- A staining buffer
- A Permeabilization Buffer
- An intracellular fixation buffer
Material
- Pipets and Pipet-aid
- Centrifugator
- Freezer
- Flow cytometer
II. Experimentation...
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