TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, their products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
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Website : www.transgenbiotech.com
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DNA transfection
DNA Transfection reagents are essential tools in molecular biology, allowing the introduction of foreign DNA into cells. This process, known as transfection, is a powerful analytical tool that enables the study of gene functions and gene products in cells. Various strategies, including biological, chemical, and physical methods, can be employed for transfection.
Chemical transfection methods, for instance, facilitate cross-membrane transport of DNA using Ca 2+ phosphate, polycations, or dendrimers. The optimal transfection method is chosen based on factors such as the type and origin of cells and the form of introduced DNA.
The successful delivery of genetic material into cells depends on the quantity and quality of the DNA, incubation time, and the ratio of transfection reagent to DNA. Advancements in life science technology now enable the transfection of various types of DNA into mammalian cells
