Reagents and instruments for immunology, cell biology and molecular biology.
 

TransGen Biotech

 

TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. 

Currently, their products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies. 
 
Read more : 

  

  

  

 

 

 

 

 

 

                     

Cell Biology 

 

  

     Mycoplasma Detection Cell Biology 

  

Natural Killer Cell Research Solution 

  

EasyPure® Fast Cell RNA Kit

 

  

Cell and Tissue Culture

 

  

TransScript® 5'/3' Race Kit

 

 

  

 

 

 

                 

Molecular Diagnostic Products

 

 

  

PerfectStart® Fast Green qPCR SuperMix

 

  

PerfectStart® II Probe qPCR SuperMix UDG

 

  

Reagent Solutions for Molecular Diagnosis

 

  

TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG

 
 
 

Watch the videos: 

 

  

  
 

 
             

TS-32 Automated Nucleic Acid Extractors

 

 

 

  

How to use SARS-CoV-2 Nucleic Acid

Detection Kit (Multiplex Real Time RT-PCR)

 

 

  

TransGen webinar The basics of Quantitative

Polymerase Chain Reaction (qPCR)

 

 

  

Cell Culture: The basic essentials you need to know
 

 

 

 
 

 
             

TransGen Webinar for NGS Products

  

TransGen-Plasmid Extraction

  

TransGen's Vlog

  

TSI 100 Nucleic Acid Gel Imaging System
 
 
 
> TransGen Biotech > Products for research > Molecular Biology Tools > Next Generation Sequencing (NGS)
Next Generation Sequencing (NGS)

Next Generation Sequencing (NGS)

Next-generation sequencing (NGS), also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies including:
  • Illumina® (Solexa) sequencing
  • Roche 454 sequencing
  • Ion torrent: Proton / PGM sequencing
  • SOLiD sequencing
These recent technologies allow us to sequence DNA and RNA much more quickly and cheaply than the previously used Sanger sequencing, and as such have revolutionised the study of genomics and molecular biology
 
Next generation methods of DNA sequencing have three general steps:
 
  • Library preparation: libraries are created using random fragmentation of DNA, followed by ligation with custom linkers
  • Amplification: the library is amplified using clonal amplification methods and PCR
  • Sequencing: DNA is sequenced using one of several different approaches
Illumina® next-generation sequencing (NGS) technology uses clonal amplification and sequencing by synthesis (SBS) chemistry to enable rapid, accurate sequencing. The process simultaneously identifies DNA bases while incorporating them into a nucleic acid chain. Each base emits a unique fluorescent signal as it is added to the growing strand, which is used to determine the order of the DNA sequence.
NGS technology can be used to sequence the DNA from any organism, providing valuable information in response to almost any biological question. A highly scalable technology, DNA sequencing can be applied to small, targeted regions or the entire genome through a variety of methods, enabling researchers to investigate and better understand health and disease.
Library preparation
Library preparation
NGS Library quantification
NGS Library quantification