TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, their products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
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Website : www.transgenbiotech.com
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![Mouse genotyping](https://www.clinisciences.com/en/upload/thumbs/mouse-genotyping-dv2jhc-bB7MAms8-51b28d.jpg)
Mouse genotyping
Accurate identification of genetically modified animals is critical for effective research and the reduction of animal usage in experimental studies. In general, genotype determination is achieved through DNA analysis, primarily from young rodent tissues. Polymerase chain reaction (PCR) analysis is the most common method used for this purpose and requires a small quantity of DNA. Tissue samples such as tail biopsies, ear perforations, hairs, blood, fecal or oral specimens can be used for PCR analysis.
The isolation of DNA from transgenic mouse samples is a challenging process that has been addressed by numerous studies with varying degrees of success. Silica membrane column purification methods can effectively eliminate contaminants and inhibitors but are costly and may have low yield. Cheaper alternatives such as sodium hydroxide (NaOH) and proteinase K (PK) can save time, but contamination can lead to inconsistent results.
To address this issue, we propose PCR genotyping kits that include the necessary reagents for DNA extraction from various samples (tail, ear, hair, blood, etc.) and the amplification of specific sequences.
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