Reagents and instruments for immunology, cell biology and molecular biology.
 

TransGen Biotech

 

TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. 

Currently, their products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies. 
 
Read more : 

  

  

  

 

 

 

 

 

 

                     

Cell Biology 

 

  

     Mycoplasma Detection Cell Biology 

  

Natural Killer Cell Research Solution 

  

EasyPure® Fast Cell RNA Kit

 

  

Cell and Tissue Culture

 

  

TransScript® 5'/3' Race Kit

 

 

  

 

 

 

                 

Molecular Diagnostic Products

 

 

  

PerfectStart® Fast Green qPCR SuperMix

 

  

PerfectStart® II Probe qPCR SuperMix UDG

 

  

Reagent Solutions for Molecular Diagnosis

 

  

TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG

 
 
 

Watch the videos: 

 

  

  
 

 
             

TS-32 Automated Nucleic Acid Extractors

 

 

 

  

How to use SARS-CoV-2 Nucleic Acid

Detection Kit (Multiplex Real Time RT-PCR)

 

 

  

TransGen webinar The basics of Quantitative

Polymerase Chain Reaction (qPCR)

 

 

  

Cell Culture: The basic essentials you need to know
 

 

 

 
 

 
             

TransGen Webinar for NGS Products

  

TransGen-Plasmid Extraction

  

TransGen's Vlog

  

TSI 100 Nucleic Acid Gel Imaging System
 
 
 
> TransGen Biotech > Products for research > Molecular Biology Tools > PCR products > Reverse transcriptase PCR - RT-PCR
Reverse transcriptase PCR - RT-PCR

Reverse transcriptase PCR - RT-PCR

RT-PCR is a technique which makes it possible to make a PCR (polymerase chain reaction) from an RNA sample. RNA is first retrotranscribed by an enzyme called reverse transcriptase, which allows the synthesis of the complementary DNA (cDNA). The latter is then used to carry out a PCR. Reverse transcriptase or reverse transcriptase (RT) is an enzyme used by retroviruses and retrotransposons that transcribe the genetic information of viruses or retrotransposons from RNA to DNA, which can integrate into the genome of the host.
RT-PCR has been developed to use RNAs as a PCR amplification matrix. It is certainly the most sensitive method for detecting (and possibly quantifying) messenger RNAs at the level of an organ, tissue or cell. For this purpose, it is often carried out in situ (PCR in situ), ie on fixed biological material. It is also used for the construction of cDNA libraries, sorting of mRNA (Differencial Display RT-PCR) as well as the construction of DNA probes.

Reverse transcriptase
Reverse transcriptase
RT-PCR Mixes and Kits
RT-PCR Mixes and Kits