Reagents and instruments for immunology, cell biology and molecular biology.

TransGen Biotech

TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research.

Currently, their products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.

Read more :

Cell Biology

Mycoplasma Detection Cell Biology

Natural Killer Cell Research Solution

EasyPure^® Fast Cell RNA Kit

Cell and Tissue Culture

TransScript^® 5'/3' Race Kit

Molecular Diagnostic Products

PerfectStart^® Fast Green qPCR SuperMix

PerfectStart^® II Probe qPCR SuperMix UDG

Reagent Solutions for Molecular Diagnosis

TransScript^® II Multiplex Probe One-Step qRT-PCR SuperMix UDG

Watch the videos:

TS-32 Automated Nucleic Acid Extractors

How to use SARS-CoV-2 Nucleic Acid

Detection Kit (Multiplex Real Time RT-PCR)

TransGen webinar The basics of Quantitative

Polymerase Chain Reaction (qPCR)

Cell Culture: The basic essentials you need to know

TransGen Webinar for NGS Products

TransGen-Plasmid Extraction

TransGen's Vlog

TSI 100 Nucleic Acid Gel Imaging System

Website : www.transgenbiotech.com

Universal SYBR Green Mixes for RT-qPCR

The universal SYBR Green quantitative PCR mixes are made for use with all types of qPCR devices.

Mixes contain SYBR Green mix (Taq polymerase, dNTPs, MgCl2 and SYBR Green) and 2 different ROX dyes: High Rox and Low Rox. Simply add the corresponding dye for the qPCR device you want to use.

The advantage of the universal SYBR Green mixes is therefore to be able to use the same mix for different devices.

Here's how to select the appropriate ROX dye for the qPCR device you are using:

Universal	All instruments
Without ROX	Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler.
Low ROX	Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P
High ROX	Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus

For RT-qPCR applications, there are two approaches: one-step RT-qPCR and two-step RT-qPCR. Each of these methods has its advantages and disadvantages. As its name implies, single-step RT-qPCR combines the synthesis of complementary DNA (cDNA) of the first strand (reverse transcript, RT) and subsequent quantitative PCR in a single reaction tube. The two-step RT-qPCR involves two distinct reactions, starting with first strand cDNA synthesis (reverse transcription-RT) and then amplifying part of the resulting cDNA by quantitative PCR in a separate tube .

One-Step RT-qPCR mixes - SYBR Green

Two-Step RT-qPCR mixes - SYBR Green

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