Reagents and instruments for immunology, cell biology and molecular biology.

TransGen Biotech

TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research.

Currently, their products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.

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Cell Biology

Mycoplasma Detection Cell Biology

Natural Killer Cell Research Solution

EasyPure^® Fast Cell RNA Kit

Cell and Tissue Culture

TransScript^® 5'/3' Race Kit

Molecular Diagnostic Products

PerfectStart^® Fast Green qPCR SuperMix

PerfectStart^® II Probe qPCR SuperMix UDG

Reagent Solutions for Molecular Diagnosis

TransScript^® II Multiplex Probe One-Step qRT-PCR SuperMix UDG

Watch the videos:

TS-32 Automated Nucleic Acid Extractors

How to use SARS-CoV-2 Nucleic Acid

Detection Kit (Multiplex Real Time RT-PCR)

TransGen webinar The basics of Quantitative

Polymerase Chain Reaction (qPCR)

Cell Culture: The basic essentials you need to know

TransGen Webinar for NGS Products

TransGen-Plasmid Extraction

TransGen's Vlog

TSI 100 Nucleic Acid Gel Imaging System

Website : www.transgenbiotech.com

Detection of cell contamination

Mycoplasma and bacterial contamination is a major concern for research laboratories (e.g. in cell biology) or biopharmaceutical laboratories (production of vaccines, monoclonal antibodies, etc.).

Mycoplasmas are the smallest self-producing microorganisms and can reproduce in extracellular space. Although they are mostly harmless to humans, other host animals and plants, some species can cause disease in humans. In particular, 6 species are a frequent cause of contamination in continuous cell lines and primary cells. Because of their size, mycoplasma are not detectable (e.g. under the microscope) and yet they profoundly alter cell cultures (metabolism, cell growth, pH, viability, membrane transport).

The main source of mycoplasma contamination is other infected cell cultures as well as culture media, reagents (fetal bovine serum, trypsin, etc.) and laboratory personnel.

Mycoplasma contaminations can cost money, time and reputation, compromise research results or impact the safety of biologic therapies. Effective strategies against mycoplasma contaminations are prevention, early detection, removal of infected material, or disposal of mycoplasma.

Early detection of bacteria in cell cultures is the only reasonable solution and the only control measure against a common problem for life science laboratories: bacterial contaminations in cell cultures.

Up to 5% of all cell cultures are contaminated with bacteria but show no visual signs of bacterial contamination, such as turbidity, sudden change in the colour of the medium (phenol red) or microscopic particles. In addition, bacteria may show resistance or lack of sensitivity to standard antibiotics (e.g. penicillin/streptomycin) and have a direct impact on cell metabolism, growth and differentiation.

Mycoplasma

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