Reagents and instruments for immunology, cell biology and molecular biology.
 

APExBIO Technology

APExBIO. As a premier provider of small molecule inhibitors/activators, compound libraries, peptides, assay kits, fluorescent labels, enzymes, modified nucleotides, and various tools for molecular biology, APExBIO empowers researchers to achieve perfection and explore the unknown. Here are some highlights:

  • Product Range: APExBIO offers a wide range of featured products, including compound libraries, fluorescent labels, enzymes, and modified nucleotides. They cover over 1,000 inhibitors/activators across 23 signaling pathways from various research areas.

  • Signaling Pathways: Detailed signaling pathway maps highlight the latest research progress, making it easier for scientists to navigate their offerings.

 


Website: www.apexbt.com

 
> APExBIO Technology > Products for research > Immunohistochemistry (IHC) products > Chromogens for IHC
Chromogens for IHC

Chromogens for IHC

 
 
Detection of antigens using a specific antibody requires a chromogen to visualize the target antigen. The type of chromogen used is determined by various factors such as:
  • The type of test
  • The level of expression of the antigen
  • If it is necessary to quantify or not the signal
  • The type of acquisition used
We offer a wide choice of specific chromogens for immunohistochemistry (IHC), for use with the 2 enzymes most used in this technique: horseradish peroxidase (HRP for Horseradish Peroxidase) and alkaline phosphatase (AP). Chromogens are chemical compounds used in IHC labeling procedures that produce a colored product that can be visualized by light microscopy. The choice of chromogen to use is directly related to the enzyme used in the test. Indeed, the enzyme will catalyze the conversion of the chromogenic substrate to produce a colored precipitate. Each enzyme can catalyze the conversion of specific substrates. Here are some examples of chromogens:
 
HRP:
  • Aminoethyl carbazole (AEC)
  • 3,3'-diaminobenzidine (DAB)
  • 3,3'5,5'-tetramethylbenzidine (TMB)
  • Emerald
AP:
  • Fast Red
  • Permanent Red
  • Mixture of nitro blue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP) (NBT / BCIP)
 
AP Chromogens
AP Chromogens
HRP Chromogens
HRP Chromogens