Reagents and instruments for immunology, cell biology and molecular biology.
 

Bachem

 

Bachem is an independent, technology-based, public biochemicals company providing full service to the pharma and biotech industry.
 
Bachem is specialized in the process development and the manufacturing of peptides and complex organic molecules as active pharmaceutical ingredients (APIs), as well as innovative biochemicals for research purposes. 
  • Bachem has more than 45 years of experience in peptide research 
  • Excellent know-how in peptide chemistry and organic synthesis (technology leadership)
  • Efficient manufacturing processes (cost leadership)
  • Bachem sets industry standards
 
Website : bachem.com

 

 
> Bachem > Products for research > Immunohistochemistry (IHC) products > Chromogens for IHC > HRP Chromogens for IHC > HRP Chromogen for IHC - DAB
HRP Chromogen for IHC - DAB

HRP Chromogen for IHC - DAB



DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).

Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling

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