Reagents and instruments for immunology, cell biology and molecular biology.
 

Biorbyt

 

Biorbyt is a UK-based supplier specializing in life science reagents. They offer a wide range of products, including antibodies, ELISA kits, small molecules, and proteins. Researchers can choose from thousands of research antibodies and reagents for various applications. Biorbyt collaborates with top scientists and scholars to develop high-quality products, covering areas such as immunology, cell biology, molecular biology, and proteinomics. Their comprehensive offerings include immune assays, protein mass spectrometry, cell culture, custom antibody services, animal model establishment, and biochemical reagents. 

Biorbyt provides valuable reagents and services for biological research. With a wide selection of antibodies, assay kits, small molecules, and proteins, Biorbyt supports scientists in their experiments. They have established stable partnerships with renowned universities, research institutes, pharmaceutical companies, biotech firms, and hospitals worldwide. Biorbyt’s commitment to high-quality products and professional technical services spans immunology, cell biology, molecular biology, proteinomics, and more.

 

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> Biorbyt > Products for research > Molecular Biology Tools > PCR products > Quantitative RT-PCR - qRT-PCR > RT-qPCR SYBR Green Mixes > Universal SYBR Green Mixes for RT-qPCR
Universal SYBR Green Mixes for RT-qPCR

Universal SYBR Green Mixes for RT-qPCR


The universal SYBR Green quantitative PCR mixes are made for use with all types of qPCR devices.
Mixes contain SYBR Green mix (Taq polymerase, dNTPs, MgCl2 and SYBR Green) and 2 different ROX dyes: High Rox and Low Rox. Simply add the corresponding dye for the qPCR device you want to use.

The advantage of the universal SYBR Green mixes is therefore to be able to use the same mix for different devices.

Here's how to select the appropriate ROX dye for the qPCR device you are using:

Universal All instruments
Without ROX Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4;
Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s;
Illumina Eco qPCR;
Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000;
Roche Applied Science LightCyclerTM 480;
Thermo Scientific PikoReal Cycler.
Low ROX Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000,
MX3005P, MX3000P
High ROX Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast;
StepOne, StepOnePlus

For RT-qPCR applications, there are two approaches: one-step RT-qPCR and two-step RT-qPCR. Each of these methods has its advantages and disadvantages. As its name implies, single-step RT-qPCR combines the synthesis of complementary DNA (cDNA) of the first strand (reverse transcript, RT) and subsequent quantitative PCR in a single reaction tube. The two-step RT-qPCR involves two distinct reactions, starting with first strand cDNA synthesis (reverse transcription-RT) and then amplifying part of the resulting cDNA by quantitative PCR in a separate tube .

RT-PCR
One-Step RT-qPCR mixes - SYBR Green
One-Step RT-qPCR mixes - SYBR Green
Two-Step RT-qPCR mixes - SYBR Green
Two-Step RT-qPCR mixes - SYBR Green

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