Reagents and instruments for immunology, cell biology and molecular biology.

Biorbyt

Biorbyt is a UK-based supplier specializing in life science reagents. They offer a wide range of products, including antibodies, ELISA kits, small molecules, and proteins. Researchers can choose from thousands of research antibodies and reagents for various applications. Biorbyt collaborates with top scientists and scholars to develop high-quality products, covering areas such as immunology, cell biology, molecular biology, and proteinomics. Their comprehensive offerings include immune assays, protein mass spectrometry, cell culture, custom antibody services, animal model establishment, and biochemical reagents.

Biorbyt provides valuable reagents and services for biological research. With a wide selection of antibodies, assay kits, small molecules, and proteins, Biorbyt supports scientists in their experiments. They have established stable partnerships with renowned universities, research institutes, pharmaceutical companies, biotech firms, and hospitals worldwide. Biorbyt’s commitment to high-quality products and professional technical services spans immunology, cell biology, molecular biology, proteinomics, and more.

Learn more :



Antibodies - Chicken Ig Why ?	Cancer Pathways Apoptosis Signaling	Fix & Perm Cell Fixation and Permeabilization


Immunology Pathways	Products for advancing discoveries	Working in larger model animal species

Watch the videos:




Recombinant Antibodies				Cancer Biomarkers

Website : https://www.biorbyt.com/

CFSE

Cytolytic activity is an important process for eliminating intracellular pathogens and cancer cells. This process is accomplished by various immune effector mechanisms, including natural killer leukocytes (NK). NK activity is facilitated by non-specific lysis of NK-receptor-infected targets, or the FcγII receptor (CD16), recognizing IgG bound to specific antigens on the target cell surface. NK cells can also induce apoptosis in target cells. The activity of natural killer cells and their effect on target cells are frequently studied in immunomodulation experiments.

Flow cytometry tests have been developed to overcome some of the difficulties associated with older assays such as lactate dehydrogenase and 51 Cr release assays.

CFSE, a green fluorescent membrane dye, is used to impart a green fluorescence characteristic to the target cell population. The unstained effector cells are then added and incubated with the target cells

At the end of the incubation, the second reagent, 7-aminoactinomycin D (7-AAD), fluorescent red dye, is added to stain all the necrotic cells by binding to the DNA of cells with membrane degeneration.

Since all target cells are initially labeled with CFSE fluorescent green and the effector cells are not, these two populations can easily be distinguished. Control populations are used to compensate for the flow cytometer. Proper synchronization of the flow cytometer easily distinguishes living and necrotic cells in a single sample tube.

Search result : 0 product found

Refine your search :

RUOCE / IVD

APPLY FILTERS
REINITIALIZE

Cat#

Description

Cond.

Price Bef. VAT

‹

›