Biorbyt

 

Biorbyt is a UK-based supplier specializing in life science reagents. They offer a wide range of products, including antibodies, ELISA kits, small molecules, and proteins. Researchers can choose from thousands of research antibodies and reagents for various applications. Biorbyt collaborates with top scientists and scholars to develop high-quality products, covering areas such as immunology, cell biology, molecular biology, and proteinomics. Their comprehensive offerings include immune assays, protein mass spectrometry, cell culture, custom antibody services, animal model establishment, and biochemical reagents. 

Biorbyt provides valuable reagents and services for biological research. With a wide selection of antibodies, assay kits, small molecules, and proteins, Biorbyt supports scientists in their experiments. They have established stable partnerships with renowned universities, research institutes, pharmaceutical companies, biotech firms, and hospitals worldwide. Biorbyt’s commitment to high-quality products and professional technical services spans immunology, cell biology, molecular biology, proteinomics, and more.

 

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Total RNA extraction - Precipitation

Total RNA extraction - Precipitation


The most common organic extraction is the method using phenol-chloroform solution
Phenol extraction is a commonly used method for removing proteins from a DNA sample, e.g. to remove proteins from cell lysate during genomic DNA preparation.
Organic extraction involves the addition of and incubation in multiple different chemical solutions; including a lysis step, a phenol chloroform extraction, an ethanol precipitation, and washing steps.
Samples are homogenized in a phenol-containing solution and then centrifuged after chloroform addition. During centrifugation, the sample separates into three phases: an upper aqueous phase which contains RNA, an interface containing DNA, and a lower organic phase containing proteins and lipids.
The advantage of the phenol-chloroform method is the simplicity of the protocol, the rapid denaturation of nucleases and the stabilization of nucleic acids. The main disadvantage is the use of toxic chemicals phenol and chloroform, and there is an increased risk of contamination due to transferring the DNA between multiple tubes.

Search result : 33 product found

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RUOCE / IVD
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  • Biochemicals 32
  • Inhibitor/Antagonist/Agonist 1
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Cat#
Description
Cond.
Price Bef. VAT
orb1298862-25mg
 25mg 
orb1298862-50mg
 50mg 
orb1298862-10mg
 10mg 
orb1298862-5mg
 5mg 
orb1220038-5mg
 5mg 
orb1298862-200mg
 200mg 
orb1298862-100mg
 100mg 
orb1298862-500mg
 500mg 
orb1700527-100mg
 100mg 
orb1700527-5mg
 5mg 
orb1700527-50mg
 50mg 
orb1703429-100mg
 100mg 
orb1703429-50mg
 50mg 
orb1703429-5mg
 5mg 
orb1745172-10mg
 10mg 
orb1745172-5mg
 5mg 
orb1745172-500mg
 500mg 
orb1745172-50mg
 50mg 
orb1745172-100mg
 100mg 
orb1745172-25mg
 25mg