Reagents and instruments for immunology, cell biology and molecular biology.

Biorbyt

Biorbyt is a UK-based supplier specializing in life science reagents. They offer a wide range of products, including antibodies, ELISA kits, small molecules, and proteins. Researchers can choose from thousands of research antibodies and reagents for various applications. Biorbyt collaborates with top scientists and scholars to develop high-quality products, covering areas such as immunology, cell biology, molecular biology, and proteinomics. Their comprehensive offerings include immune assays, protein mass spectrometry, cell culture, custom antibody services, animal model establishment, and biochemical reagents.

Biorbyt provides valuable reagents and services for biological research. With a wide selection of antibodies, assay kits, small molecules, and proteins, Biorbyt supports scientists in their experiments. They have established stable partnerships with renowned universities, research institutes, pharmaceutical companies, biotech firms, and hospitals worldwide. Biorbyt’s commitment to high-quality products and professional technical services spans immunology, cell biology, molecular biology, proteinomics, and more.

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Website : https://www.biorbyt.com/

NGS Library preparation

Next Generation Sequencing (NGS) requires careful preparation of the genetic material prior to the sequencing reaction to ensure reliable results.

The preparation of DNA banks is composed of 4 main steps whose sequence may vary depending on the methods used:

1. Fragmentation

DNA is fragmented either enzymatically or by sonication to create small random fragments.

2. End repair

Since DNA fragmentation does not result in homogeneous, open-ended fragments, end repair is necessary to ensure that each molecule is free of overhangs and contains 5' phosphate and 3' hydroxyl groups.

3. Binding of adapters

The adapters (small sequences of double-stranded synthetic DNA) are then bound to these fragments with the help of a DNA ligase. The adaptors allow the sequence to bind to the support but also to identify the sample analyzed thanks to the indexes. The adapters contain motifs necessary for the following steps (clonal amplification and sequencing)

4. Amplification of the bank

Amplification of the bank is necessary so that the signal received by the sequencer is strong enough to be detected accurately.

For the preparation of RNA libraries, a reverse transcription step must be added in order to obtain cDNA libraries.

DNA

RNA