Reagents and instruments for immunology, cell biology and molecular biology.

Biorbyt

Biorbyt is a UK-based supplier specializing in life science reagents. They offer a wide range of products, including antibodies, ELISA kits, small molecules, and proteins. Researchers can choose from thousands of research antibodies and reagents for various applications. Biorbyt collaborates with top scientists and scholars to develop high-quality products, covering areas such as immunology, cell biology, molecular biology, and proteinomics. Their comprehensive offerings include immune assays, protein mass spectrometry, cell culture, custom antibody services, animal model establishment, and biochemical reagents.

Biorbyt provides valuable reagents and services for biological research. With a wide selection of antibodies, assay kits, small molecules, and proteins, Biorbyt supports scientists in their experiments. They have established stable partnerships with renowned universities, research institutes, pharmaceutical companies, biotech firms, and hospitals worldwide. Biorbyt’s commitment to high-quality products and professional technical services spans immunology, cell biology, molecular biology, proteinomics, and more.

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Website : https://www.biorbyt.com/

Kits for the preparation of DNA libraries for NGS

Library preparation for Illumina is composed of 4 main steps, the progress of which can vary according to the methods used:

1. DNA fragmentation

DNA is fragmented either enzymatically or by sonication to create smaller strands

2. End repair

Because DNA fragmentation does not result in homogeneous, blunt-ended fragments, end repair is needed to ensure that each molecule is free of overhangs, and contains 5' phosphate and 3' hydroxyl groups

3. Adapter ligation

Adaptors (short, double-stranded pieces of synthetic DNA) are then ligated to these fragments with the help of DNA ligase. The adaptors enable the sequence to become bound to a complementary counterpart. These adapters contain sequence motifs that are required for subsequent steps (clonal amplification and the actual sequencing).

4. Library amplification

Library amplification is required so that the received signal from the sequencer is strong enough to be detected accurately. The Illumina technology uses the "Bridge PCR" method.

Bi-Seq

ChIP-Seq

Difficult samples

Genome

Single Cell