Reagents and instruments for immunology, cell biology and molecular biology.

Biorbyt

Biorbyt is a UK-based supplier specializing in life science reagents. They offer a wide range of products, including antibodies, ELISA kits, small molecules, and proteins. Researchers can choose from thousands of research antibodies and reagents for various applications. Biorbyt collaborates with top scientists and scholars to develop high-quality products, covering areas such as immunology, cell biology, molecular biology, and proteinomics. Their comprehensive offerings include immune assays, protein mass spectrometry, cell culture, custom antibody services, animal model establishment, and biochemical reagents.

Biorbyt provides valuable reagents and services for biological research. With a wide selection of antibodies, assay kits, small molecules, and proteins, Biorbyt supports scientists in their experiments. They have established stable partnerships with renowned universities, research institutes, pharmaceutical companies, biotech firms, and hospitals worldwide. Biorbyt’s commitment to high-quality products and professional technical services spans immunology, cell biology, molecular biology, proteinomics, and more.

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Antibodies - Chicken Ig Why ?	Cancer Pathways Apoptosis Signaling	Fix & Perm Cell Fixation and Permeabilization


Immunology Pathways	Products for advancing discoveries	Working in larger model animal species

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Recombinant Antibodies				Cancer Biomarkers

Website : https://www.biorbyt.com/

Sequencing of total RNA by NGS - Library preparation - Modules

Total RNA is composed of large amounts of unwanted transcripts, such as ribosomal RNA (rRNA), which accounts for 80-98% of a total RNA sample, and globin mRNA, which accounts for 35-80% of the mRNA in blood samples. Ribosomal RNA is a type of non-coding RNA that is the main component of ribosomes, essential to all cells. Ribosomal RNA is transcribed from ribosomal DNA (rDNA) and then bound to ribosomal proteins to form small and large ribosomal subunits. rRNA is the physical and mechanical component of the ribosome that forces the transfer RNA (tRNA) and messenger RNA (mRNA) to process and translate them into proteins.

Depletion of ribosomal RNA (rRNA) before RNA sequencing (Seq-RNA) is therefore essential and allows us to focus on the analysis of the portions of interest in the transcriptome. The use of oligo dT primer to capture the polyadenylated 3′ tail of transcripts and isolate the mRNA is common in many RNA sequencing preparations; however, this method does not allow processing of degraded samples where most of the RNA is separated from the 3′ tail, nor does it allow isolation of non-polyadenylated transcripts such as ncRNAs. Ribosomal removal methods address these problems by directly depleting rRNA while leaving other transcripts intact.