Biorbyt
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Biorbyt is a UK-based supplier specializing in life science reagents. They offer a wide range of products, including antibodies, ELISA kits, small molecules, and proteins. Researchers can choose from thousands of research antibodies and reagents for various applications. Biorbyt collaborates with top scientists and scholars to develop high-quality products, covering areas such as immunology, cell biology, molecular biology, and proteinomics. Their comprehensive offerings include immune assays, protein mass spectrometry, cell culture, custom antibody services, animal model establishment, and biochemical reagents. Biorbyt provides valuable reagents and services for biological research. With a wide selection of antibodies, assay kits, small molecules, and proteins, Biorbyt supports scientists in their experiments. They have established stable partnerships with renowned universities, research institutes, pharmaceutical companies, biotech firms, and hospitals worldwide. Biorbyt’s commitment to high-quality products and professional technical services spans immunology, cell biology, molecular biology, proteinomics, and more.
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Website : https://www.biorbyt.com/
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![F(ab')2 fragment secondary antibodies](https://www.clinisciences.com/en/upload/image-39-yxyy5n.png)
F(ab')2 fragment secondary antibodies
Secondary antibodies can be classified based on their specificity, which can be broad or narrow, and their fragment type, which can be whole, F(ab), or F(ab')2.
The principle of F(ab')2 is to generate divalent antibody fragments that retain the antigen-binding Fab portions of whole IgG antibodies while removing most of the Fc region.
F(ab')2 fragments are generated by pepsin digestion of whole IgG antibodies to cleave the hinge region and leave intact some of the Fc region. The resulting fragments have two antigen-binding Fab portions linked together by disulfide bonds, making them divalent with a molecular weight of about 110 kDa. The use of F(ab')2 fragments has several advantages over whole IgG antibodies, including better penetration into tissues, reduced nonspecific binding to Fc receptors on live cells or to Protein A/G, and the ability to precipitate antigens. However, F(ab')2 fragments are not recommended for blocking since they have two binding sites that are available to capture the primary antibody introduced subsequently. The preparation of F(ab')2 fragments involves pepsin digestion of whole IgG antibodies, followed by purification using techniques such as FPLC with DEAE-
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