bioWORLD

 

bioWORLD is a global leader in manufacturing and supplying a wide range of products for life science research. With over 25 years of experience, they have focused on providing quality products and excellent customer service. Their diverse customer base includes hospitals, public health institutions, commercial laboratories, biotechnology companies, pharmaceutical firms, universities, and research institutions.

 
 
IHC buffers - Quenching - AP

IHC buffers - Quenching - AP


Chromogenic detection methods often use a conjugated enzyme to visualize epitope-antibody interactions. When using this method of detection, the endogenous activity of the same enzyme must be blocked. For example, protocols that include horseradish peroxidase (HRP) or alkaline phosphatase (AP) may require reagents to prevent non-specific signals. Tissues such as kidney, liver, or vascular areas with red blood cells, contain endogenous peroxidase activity. Peroxidase blocking reagents formulated with 3-10% H2O2 can be used to prevent endogenous peroxidase from cleaving the substrate. Endogenous AP found in intestine, kidney, lymphoid and other tissue can be blocked with 1 mM Levamisole. The intestinal form of AP is unaffected by Levamisole but can be blocked by using 1% acetic acid.

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