bioWORLD
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bioWORLD is a global leader in manufacturing and supplying a wide range of products for life science research. With over 25 years of experience, they have focused on providing quality products and excellent customer service. Their diverse customer base includes hospitals, public health institutions, commercial laboratories, biotechnology companies, pharmaceutical firms, universities, and research institutions. Website : www.bio-world.com
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![Hippeastrum hybrid lectin (HHL)](https://www.clinisciences.com/en/upload/thumbs/hippeastrum-hybrid-lectin-hhl-jcsndh-2d6302.png)
Hippeastrum hybrid lectin (HHL)
Hippeastrum hybrid lectin (HHL) isolated from amaryllis bulbs. It consists of four subunits of identical size and has a molecular weight of 50,000. HHL has an isoelectric point between pH 4.7 and pH 5.1. It only binds alpha-mannose residues, (alpha-1,3) and (alpha-1,6) linked mannosyl units, as well as some yeast galactomannans. HHL has a blood group specificity for rabbit.
Study shows when treatment of the linear alpha-1,3-mannan with periodate, resulting in oxidation of the terminal, nonreducing mannosyl group, reactivity with HHL was not reduced. Oligosaccharides containing either 1,3- or 1,6-linked mannosyl units are good inhibitors of HHL-mannan precipitation system. Observations suggests that HHL reacts not only with terminal but also with internal D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked mannosyl units. Those containing alpha-1,3- or alpha-1,6-mannosyl residues were retarded on immobilized HHL agarose column.
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- Agarose 2
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