Reagents and instruments for immunology, cell biology and molecular biology.
 

MedChemExpress

MCE offers over 40,000 small molecule inhibitors or agonists targeting more than 1,000 targets across 20+ signaling pathways, encompassing various hot research areas such as cancer, neurological diseases, and cardiovascular diseases.

Natural Products

 

Labeled Compounds

 

Compound Library
MCE provides you with over 110 00+ natural products, covering a variety of structural classifications and product sources , which can be applied in industries such as scientific research experiments, food, and cosmetics. In addition, not only can you filter out the required products according to your needs, but they also provide product customization services, which can fully assist your scientific research. Isotope  

MCE can provide over 7700 high purity, high isotopic enrichment compounds, which include 2 H, 13 C, 15 N, 18 O, and other stable isotope atoms. These can be used as tracers for metabolic flow studies, or as quantitative internal standards for techniques such as NMR, LC/GC MS, LC/GC MS/MS, etc.

 

  
MCE supplies more than 200+ compound libraries, including bioactive compound libraries, diversity compound libraries, fragment libraries, as well as diversified customized libraries based on your special needs. Those libraries can be used for drug repurposing, new drug discovery and mechanism research. Besides, MCE also provides target based compound screening (kinase, GPCR, nuclear receptor, ion c hannel, etc.), cell phenotype based compound screening (cell viability, apoptosis, etc.), affinity based compound screening (affinity mass spectrometry screening, DEL synthesis and screening), molecular interactions assays (SPR, BLI, ITC, MST, etc.), and customized screening, fully assisting you in drug discovery.
         

PROTAC MCE

 

Reference Standards MCE

 

GMP Small M olecules

MCE can provide a series of PROTAC related products, such as Ligand for E3 Ligase 、 PROTAC Linker 、 Ligand for Target Protein for PROTAC E3 Ligase Ligand Linker Conjugate Target Protein Ligand Linker Conjugate PROTAC, etc.

Those products can be used for PROTAC synthesis. Besides, MCE also provides one stop services for the design, synthesis, analysis, purification, optimization, detection and evaluation of PROTAC related products mentioned above , fully assisting you in PROTAC drug

  


Offers a wide variety of reference standards (analytical standards), including active small molecules, natural products, lipids, isotopes (Internal standards), and more. These products are applicable in fields including pharmaceutical analysis, identification and quantification of biomarkers, environmental monitoring, and food safety. All reference standards MCE supplied are rigorously tested under the ISO 17025 system. Comprehensive an alytical documentation accompanies each product, providing evidence of purity, content, and structure, supporting measurement traceability.

  
MCE offers 60+ GMP related products covering applications in cell and gene therapy and antibody drug conjugates. GMP small molecule compounds are auxiliary reagents in cell therapy production and can be used for the reprogramming, expansion and differentiation of stem cells. Those products can be used in gene therapy to enhance de livery efficiency, etc. GMP ADC related small molecule products include ADC Payload, Drug Linker Conjugates and related intermediates. The GMP small molecule products provided by MCE are produced in strict compliance with cGMP guidelines, with reliable qua lity and high cost performance. They support declaration work and on site audits from IND to NDA.
         

ADC Related

 

Oligonucleotides

 

Recombinant Proteins

MCE provides 1600+ ADC related products, including ADC Antibody, ADC Cytotoxin, ADC Linker, Antibody Drug Conjugates (ADCs), and Drug Linker Conjugates for ADC. In addition to ADC, they also provide related product customization and synthesis services for other conjugated drugs PROTAC Linker Conjugates for PAC, ApDC, AOC, PDC, FDC, RD C, ISAC, VDC and SMDC. It is used in cell and animal experiments (mice, rats, macaques, cynomolgus monkeys, patients, etc.) to study the killing effect on tumors and pave the way for new cancer treatments.		  
MCE offers over 30,000 oligonucle otides, including antisense oligonucleotides (ASO), siRNA, miRNA, and nucleic acid aptamers, covering various chemical modifications (phosphorothioation, 2' F, 2' OMe, and 2' MOE, fluorophores (such as Cy3, Cy5, and FAM)). MCE has a professional team of re searchers, producers, and analysts specializing in oligonucleotide synthesis, with production capabilities ranging from micrograms to kilograms, providing oligonucleotides tailored to customer needs.		   MCE offers 10,000+ recombinant proteins with excellent lot to lot consistency, superior activity, and low endotoxin levels. Additionally, They provide personalized customization services. These products can be applied in cell culture, structure and function analysis, immunology assays, antibody production, and in vitro biochemical analysis, among other applications.
         

Antibodies

 

Inhibitory Antibodies

 

Enzymes

 

MCE provides over 4000 high quality primary antibodies and a variety of superior secondary antibodies, comprehensively covering signaling pathways such as autophagy, apoptosis, immunity, and the cell cycle, with a deep focus on major popular targets. In addition, MCE has devoted itself to the development of over 3300 premium monoclonal antibodies, which are more specific and sensitive, comprehensively empowering your scientific research!

   MCE provides 1200+ high quality, high efficiency and high purity in vivo Inhibitory Antibodies, which can be used as positive controls for drug effect evaluation and other related scientific research. MCE's neutralizing/blocking/functional inhibitory antibodies are widely used in immunology, oncology, neurology, and other popular research fields to help preclinical studies!   MCE provides 3000 enzyme products in a comprehensive range, including Glycobio logy Enzymes, Disease Research enzymes, tissue dissociation enzymes, nucleases, proteases, biochemical detection enzymes, tool enzymes, coenzymes and others. There are also a variety of recombinases covering multiple targets.
         

Peptides

 

Biochemical Assay Reagents

 

Dyes
MCE provides a comprehensive collection of high quality peptides including tag peptides, therapeutics peptides, cell penetrating peptides and amino acid derivatives, which have been widely used in functional analysis, antibody research, vaccine research, and especially the field of drug development. Our custom peptide service is also available to propel your projects.  

MCE offers a comprehensive range of 3 000+ high quality biochemical assay reagents, including co solvents, chelators, cell assay reagen ts, drug delivery reagents, buffer reagents, etc. Starting from dissolution, the first step of the experiment, MCE biochemical assay reagents can meet your experimental needs and fully assist your experiments.

  
MCE provides 2 000+ high quality dye re agents covering many popular research fields, generally with high photon yield, and high signal intensity F luorescent signals can appear within a short time after dyeing, and are not easily affected by light, PH, temperature and fixing agent, and their applications have been deeply penetrated into many fields such as pharmacology, physiology, environmental science, information science, etc., and they have been widely applied to the functional study of proteins, drug screening and other fields. It is also widely used in the fields of protein function research and drug screening. 
         

Kits MCE

 

Virtual Screening

 

Compound Screening

Offers a broad range of high quality, cost effective, ready to use assay kits for the convenient and dependable analysis of molecular, protein, and cell biology research. These assay kits range from PCR & qPCR & RT PCR, nucleic acid electrophoresis to vector construction, from protein preparation, protein purification to protein detection, from cell culture to cell analysis.

  

Virtual Screening (VS) is a computational approach for identifying active compounds from small molecule databases. MCE, with its extensive database resources and high performance computing servers, offers professional molecular docking and virtual screening services.

 

  
MCE provides target based compound screening (kinase, GPCR, nuclear receptor, ion channel, etc.), cell phenotype based compound screening (cell viability, apoptosis, etc.), affinity based compound screening (affinity mass spectrometry screening, DEL synthesis and screening), molecular interactions assays (SPR, BLI, ITC, MST, etc.), and customized screening, fully assisting you in drug discovery.

 

Learn more about MCE cytokines: Ideal growth factors for your research

 

 

MCE provides a one-stop solution for drug screening to accelerate early drug discovery! wach the video 

 


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> MedChemExpress > Products for research > Molecular Biology Tools > PCR products > Conventional PCR
Conventional PCR

Conventional PCR



The polymerase chain reaction (PCR) is a test tube system for DNA replication which allows a “target” DNA sequence to be selectively amplified several million fold in just a few hours. The PCR achieves amplification of a predetermined fragment of DNA, (the target; which can e.g. be from 100 – 1000 bp long)

A basic PCR set up requires several components and reagents. These components include:
  • DNA template that contains the DNA region (target) to amplify
  • Taq polymerase, a DNA polymerase that is heat resistant, so that it can remain intact during the DNA denaturation process.
  • Two primers that are complementary to the 3' ends of each of the sense and anti-sense strand of the DNA target (DNA polymerase can only bind and elongate from a double-stranded region of DNA, and without primers there is no double-stranded initiation site for polymerase to bind).
  • Deoxynucleoside triphosphates (dNTPs, sometimes called "deoxynucleotide triphosphates"; nucleotides containing triphosphate groups), the building-blocks from which the DNA polymerase synthesizes a new DNA strand.
  • Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase
  • Bivalent cations, magnesium or manganese ions; generally Mg2+ is used, but Mn2+ can be used for PCR-mediated DNA mutagenesis, as higher Mn2+ concentration increases the error rate during DNA synthesis
  • Monovalent cation potassium ions

Typically, PCR consists of a series of 20–40 repeated temperature changes, called cycles, with each cycle commonly consisting of 2–3 discrete temperature steps. The cycling is often preceded by a single temperature step at a high temperature (>90 °C), and followed by one hold at the end for final product extension or brief storage. The temperatures used and the length of time they are applied in each cycle depend on a variety of parameters. These include the enzyme used for DNA synthesis, the concentration of divalent ions and dNTPs in the reaction, and the melting temperature (Tm) of the primers.

  • Initialization step (Only required for DNA polymerases that require heat activation by hot-start PCR): This step consists of heating the reaction to a temperature of 94–96 °C (or 98 °C if extremely thermostable polymerases are used), which is held for 1–9 minutes.
  • Denaturation step: This step is the first regular cycling event and consists of heating the reaction to 94–98 °C for 20–30 seconds. It causes DNA melting of the DNA template by disrupting the hydrogen bonds between complementary bases, yielding single-stranded DNA molecules.
  • Annealing step: The reaction temperature is lowered to 50–65 °C for 20–40 seconds allowing annealing of the primers to the single-stranded DNA template.
  • Extension/elongation step: The temperature at this step depends on the DNA polymerase used; Taq polymerase has its optimum activity temperature at 75–80 °C and commonly a temperature of 72 °C is used with this enzyme. At this step the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding dNTPs that are complementary to the template in 5' to 3' direction, condensing the 5'-phosphate group of the dNTPs with the 3'-hydroxyl group at the end of the nascent (extending) DNA strand. The extension time depends both on the DNA polymerase used and on the length of the DNA fragment to amplify.

DNA polymerases
DNA polymerases
Reagents for PCR
Reagents for PCR