β - galactosidase (β –GAL) Activity Assay Kit
Cat# E-BC-K631-M-96T
Size : 96T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
Detection principle
β-galactosidase can catalyze the substrate nitrophenylgalactoside pyranopyranoside to produce nitrophenol, which has a maximum absorption peak at 400 nm. The activity of β-GAL can be calculated by measuring the OD value at 400 nm.
Performance characteristics
| Synonyms | β-GAL |
| Sample type | cells, animal and plant tissue |
| Sensitivity | 16.19 U/L |
| Detection range | 16.19-1200 U/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 70 min |
| Precision | Average inter-assay CV: 6%Average intra-assay CV: 3% |
| Other instruments required | Water bath, Incubator, Centrifuge, Microplate reader |
| Other reagents required | 2 mmol/L hydrochloric acid |
| Storage | Reagent 2: -20℃; Others: 2-8℃ |
| Valid period | 12 months |
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits