ADH1 Rabbit Polyclonal Antibody

Cat# R1049BS

Size : 100ug

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Specifications
Product Data
Application ELISA, IP, WB
Application Western blot: 1/500-1/5,000.
Immunoprecipitation: 1/100.
ELISA: 1/5,000-1/20,000.
Antibody has been assayed against 1.0 ug of Alcohol dehydrogenase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
Reactivity Yeast
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Alcohol dehydrogenase from yeast
Specificity This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined.
Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase.
Buffer 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: Biotin
State: Lyophilized purified Ig faction
Stabilizer: 10 mg/m BSA (immunoglobulin and protease free)
Preservative: 0.01% Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration lot specific
Purification Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Database Link
Synonyms Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947
Reference Data
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