Reagents and instruments for immunology, cell biology and molecular biology.

Akt1, phosphorylated (Ser473) (Rac PKa, PKBa) (Biotin)

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Cat# A1125-03-Biotin-100ul

Size : 100ul

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Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

A1125-03-Biotin Akt1, phosphorylated (Ser473) (Rac PKa, PKBa) (Biotin)

Clone Type

Polyclonal

Host

rabbit

Source

human

Swiss Prot

P31749

Isotype

IgG

Grade

Affinity Purified

Applications

Crossreactivity

Bo Ch Hu Mo Rt Xe

Accession #

NM_005163

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications: |Suitable for use in Western Blot. Other applications not tested. ||Recommended Dilution:|Western Blot Analysis: 0.07-0.4ug/ml of this lot detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 50ng/ml PDGF for 20 minutes.|Optimal dilutions to be determined by the researcher. ||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG|Clone No: SK703|Host: rabbit|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.|Purity: Purified by Protein A affinity chromatography.|Immunogen: KLH-conjugated, synthetic peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa. The immunizing|sequence is identical in mouse and bovine Akt1. Akt2 and Akt3 share significant homology with the peptide immunogen sequence.|Specificity: Recognizes human Akt1/PKBa when phosphorylated at Ser473. Crossreactivity to phosphorylated Akt2 and Akt3 likely based on sequence homology. Species Crossreactivity: mouse. Predicted crossreactivity with rat, bovine, chicken, and Xenopus based on immunogen sequence homology.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

KLH-conjugated, synthetic peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa. The immunizing|sequence is identical in mouse and bovine Akt1. Akt2 and Akt3 share significant homology with the peptide immunogen sequence.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes human Akt1/PKBa when phosphorylated at Ser473. Crossreactivity to phosphorylated Akt2 and Akt3 likely based on sequence homology. Species Crossreactivity: mouse. Predicted crossreactivity with rat, bovine, chicken, and Xenopus based on immunogen sequence homology.

References

11. Cross, D.A., et al., Nature 378: 785-789, 1995. 2. James, S.R., et al., Biochem. J. 315: 709-713, 1996. 3. Alessi, D.R., et al., Curr. Biol. 8: 69-81, 1998. 4. Alessi, D.R., et al., Curr. Biol. 7: 776-789, 1997. 5. Cohen, P., et al., FEBS Lett. 410: 3-10, 1997. General References:1. Spieker-Polet H, et al. Proc Natl Acad Sci. 1995 Sep 26;92(20):9348-52. 2. Liguori MJ, et al. Hybridoma. 2001 Jun; 20(3):189-98. 3. G.Cano1, F. Milanezi2, D. Leitao2,3, S. Ricardo2, M.J. Brito1, F. C. Schmitt2-3 1Garcia da Orta Hospital, Almada, Portugal,2 Inst. Molec. Pathology and Immunology of Porto University, Portugal,3 Medical Faculty of Porto university, Portugal Diagn Cytopathol, 2003 Oct; 29(4): 207 -11. 4. L.K. Diaz* and N.Sneige *Department of Pathology,Northwestern University, Chicago,+ Department of Pathology, University of Texas, Huston, Adv Anat Pathol,2005; 12(1), 10-19. 5. Z. Huang1, W. Zhu2, G. Szekeres3, H. Xia1 1Spring Bioscience Corp, Fremont,CA, 2 Epitomics Inc, Burlingame,CA, , 4Histopathology Ltd, Hungary, Appl Immunohistochem Mol Morphol. 2005; 13 (1): 91-95 6. S. Rossi1, E. Orvieto1, S.Chinellato1, A. Furlanetto1, L.Laurino1, F. Facchetti2, AP Dei Tos 2 1Department of Pathology, 2Treviso, Italy; *Brescia, University School of Medicine, Brescia, Italy., Abstract presented at USCAP 2004. Modern Pathology 2004; 17 (suppl 1): 361A 7. M. Blechner, E. Ballesteros, D. Mandich, D. Stevens, R. Cartun, Hartford Hospital, Hartford, CT. Abstract presented at USCAP 2004. Modern Pathology 2004; 17 (suppl 1): 241A 8. W. Cheuk, K.O.Y. Wong, C.S.C. Wong and J.K.C. Chan, Department of Pathology, Queen Elizabeth Hospital, Hong Kong, Am J Surg Path, 2004; 28 (6): 801-807. 9. G.B. Budd, E. Tso, B. Yoder, T. Choueiri, P. Elson, S. Tarr, M. Skacel, R. Tubbs, A. Dawson, D. Hicks, Cleveland Clinic Foundation, Cleveland, OH, Abstract presented at ASCO Annual meeting, June 2004, New Orleans 10. S. M. Tarr, S. Short, K. Hansen, T. Morken, H. Xia, E. Downs-Kelly, R. R. Tubbs, D. G. Hicks, Department of Pathology and Laboratory Medicine. The Cleveland Clinic Foundation, Cleveland, Ohio. Lab Vision Corp., Fremont, Ca., Spring Bioscience Corp, Fremont ,CA, Abstract presented at Association for Molecular Pathology meeting, Los Angeles, 2004 11. A.M. Gown, T.S. Barry, P. Kandalaft, L.C. Goldstein, C.C. Tse and D.O. Treaba, Clinical Research Division , PhenoPath Laboratories and IMPRIS, Seattle, WA, Abstract presented at USCAP 2005. Modern Pathology 2005; 18, suppl.1,pag 35A 12. D.O. Treaba, A.W. Hing, L.C. Goldstein, T.S. Barry, P. Kandalaft, C.B. Gilks, T.O. Nielsen and A.M. Gown, Clinical Research Division , PhenoPath Laboratories and IMPRIS, Seattle, WA, USA Genetic Pathology Evaluation Centre, University of British Columbia, Vancouver, BC, Canada, Abstract presented at USCAP 2005. Modern Pathology 2005; 18, suppl.1,pag 53A 13. S. Rossi1, L. Laurino1, A. Furlanetto1, S.Chinellato1, E. Orvieto1, F. Canal1, F. Facchetti2, A.P. Dei Tos1 1 Depart. Pathology, Hospital of Treviso, Italy, 2 Brescia University School of Medicine, Brescia, Italy, Am J Clin Pathol, 2005, Aug;124(2):295-302