This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/4,000).
Reactivity
Equine
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Alcohol dehydrogenase isolated and purified from Horse Liver. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes Alcohol Dehydrogenase.
Buffer
PBS, pH 7.2 without preservatives and foreign proteins. State: Azide Free State: Lyophilized IgG fraction.
Reconstitution Method
Restore by adding 1.0 ml of sterile distilled water.
Concentration
lot specific
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation
Unconjugated
Storage
Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Stability
Shelf life: one year from despatch.
Shipping
Ambient
Background
ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
