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> Alkyne Cholesterol [1631985-09-5]

Alkyne Cholesterol [1631985-09-5]

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Cat# HY-151705-5mg

Size : 5mg

Brand : MedChemExpress

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Phone : +1 850 650 7790

Alkyne Cholesterol, a modified lipid, is a click chemistry reagent containing an alkyne group. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents in the presence of a copper (Cu)-containing catalyst. Alkyne Cholesterol can be used for tracking cellular cholesterol metabolism and localization.

For research use only. We do not sell to patients.

Alkyne Cholesterol Chemical Structure

Alkyne Cholesterol Chemical Structure

CAS No. : 1631985-09-5

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Description

Alkyne Cholesterol, a modified lipid, is a click chemistry reagent containing an alkyne group. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents in the presence of a copper (Cu)-containing catalyst. Alkyne Cholesterol can be used for tracking cellular cholesterol metabolism and localization[1][2][3].

Molecular Weight

368.60

Formula

C26H40O
CAS No.

1631985-09-5
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](CC[C@]2(22)[C@H](C)CCCC#C)(22)[C@@]3(22)[C@@](CC1)(22)[C@@]4(C(C[C@H](CC4)O)=CC3)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 4 mg/mL (10.85 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7130 mL 13.5648 mL 27.1297 mL
5 mM 0.5426 mL 2.7130 mL 5.4259 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥95.0%

COA (247 KB) HNMR (104 KB)

Handling Instructions (2659 KB)
References

  • [1]. Katherine Windsor, et al. Probing lipid-protein adduction with alkynyl surrogates: application to Smith-Lemli-Opitz syndrome. J Lipid Res. 2013 Oct;54(10):2842-50.  [Content Brief]

    [2]. Katherine Windsor, et al. Assay of protein and peptide adducts of cholesterol ozonolysis products by hydrophobic and click enrichment methods. Chem Res Toxicol. 2014 Oct 20;27(10):1757-68.  [Content Brief]

    [3]. Alkyne Cholesterol.

  • [1]. Katherine Windsor, et al. Probing lipid-protein adduction with alkynyl surrogates: application to Smith-Lemli-Opitz syndrome. J Lipid Res. 2013 Oct;54(10):2842-50.

    [2]. Katherine Windsor, et al. Assay of protein and peptide adducts of cholesterol ozonolysis products by hydrophobic and click enrichment methods. Chem Res Toxicol. 2014 Oct 20;27(10):1757-68.

    [3]. Alkyne Cholesterol.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7130 mL 13.5648 mL 27.1297 mL 67.8242 mL
5 mM 0.5426 mL 2.7130 mL 5.4259 mL 13.5648 mL
10 mM 0.2713 mL 1.3565 mL 2.7130 mL 6.7824 mL
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Alkyne Cholesterol Related Classifications

  • Click Chemistry
  • Alkynes Labeling and Fluorescence Imaging
  • Biochemical Assay Reagents
  • Drug Delivery
  • Metabolic Disease
  • Metabolic Enzyme/Protease
  • Liposome
Help & FAQs

Keywords:

Alkyne Cholesterol1631985-09-5Liposomeclick chemistryreagentalkynecholesterolmetabolismlocalizationInhibitorinhibitorinhibit