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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-ACTB antibody (Clone: 5B7)

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Cat# NB-22-6928-50

Size : 50ug

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Phone : +1 850 650 7790

Brand : Neo Biotech

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Contact local distributor :

Phone : +1 850 650 7790

Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) , Chicken lung (4) and Rabbit testis (5), diluted at 1:10000.
IHC Staining of Human ovary tissue, diluted at 1:200
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Beta-actin monoclonal antibody (5B7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, Beta-actin monoclonal antibody (5B7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, Beta-actin monoclonal antibody (5B7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.
Immunofluorescence analysis of Hela cell. 1, Cyclin A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-actin monoclonal antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

General Info

Host:	Mouse
Applications:	IF/WB/IHC
Reactivity:	Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect/Pig/Lenok
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Actin, cytoplasmic 1 is suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry research applications.

Clonality:	Monoclonal
Clone ID:	5B7
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	IF 1:50-200 WB 1:5000-20000 IHC 1:200-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	ACTB
Gene ID:	60
Uniprot ID:	ACTB_HUMAN
Specificity:	The antibody detects endogenous Beta-actin protein.
Immunogen:	Synthetic Peptide of Beta-actin

Description

Function	Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules.
Protein Name	Actin - Cytoplasmic 1 Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed
Database Links	Reactome: R-HSA-1445148 Reactome: R-HSA-190873 Reactome: R-HSA-196025 Reactome: R-HSA-2029482 Reactome: R-HSA-3214847 Reactome: R-HSA-389957 Reactome: R-HSA-390450 Reactome: R-HSA-3928662 Reactome: R-HSA-3928665 Reactome: R-HSA-418990 Reactome: R-HSA-437239 Reactome: R-HSA-4420097 Reactome: R-HSA-445095 Reactome: R-HSA-446353 Reactome: R-HSA-5250924 Reactome: R-HSA-5626467 Reactome: R-HSA-5663213 Reactome: R-HSA-5663220 Reactome: R-HSA-5674135 Reactome: R-HSA-5689603 Reactome: R-HSA-5696394 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-8856828 Reactome: R-HSA-9035034 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9662360 Reactome: R-HSA-9662361 Reactome: R-HSA-9664422 Reactome: R-HSA-983231
Cellular Localisation	Cytoplasm Cytoskeleton Nucleus Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names	Anti-Actin - Cytoplasmic 1 antibody Anti-Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed antibody Anti-ACTB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance