Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue using Anti-beta actin Rabbit Monoclonal Antibody, Clone RM112, at a 1:1000 dilution. Immunocytochemical staining of HeLa cells, using anti-β-Actin RevMAb Clone RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue). Western blot of A431 cells, using anti-β-Actin RevMAb Clone RM112 at a 1/1000 dilution, showed a band of β-Actin at the predicted MW (42 kDa).
Product Details Product Name Anti-β-Actin Rabbit Monoclonal Antibody, Clone RM112 Description Rabbit monoclonal to β-Actin (beta-actin) Catalog No. 31-1013-00 Clone Name RM112 Product Data Sheet Anti-Beta Actin Rabbit Monoclonal Antibody Data Sheet RM112 Specificity RM112 reacts to β-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin. Immunogen A peptide corresponding to the N-terminus of β-actin Application Western Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity All Species of Animals Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot (WB): 1:1000 dilution;
Immunoprecipitation (IP): 1:200 dilution;
Chromatin IP (ChIP): 1:200 dilution;
Immunocytochemistry (ICC): 1:200 dilution;
Immunohistochemistry (IHC): 1:200-1:1000 dilution. Keywords References for β-Actin Rabbit Monoclonal Antibody [RM112] Jevtić P et al. The nucleoporin ELYS regulates nuclear size by controlling NPC number and nuclear import capacity. EMBO Rep. (2019), 10.15252/embr.201847283. WB; Human. Read more (PubMed: 31085625)
Wang G et al. Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. Elife. (2019), 10.7554/eLife.38730. WB; Human. Read more (PubMed: 31042147)
Mukherjee RN et al. Reticulon 4a promotes exocytosis in mammalian cells. Elife. (2019), 10.1091/mbc.E19-03-0159. WB; Human. Read more (PubMed: 31318320)
Product Images
Anti-β-Actin rabbit monoclonal antibody [RM112]
![Anti-β-Actin rabbit monoclonal antibody [RM112]](https://www.clinisciences.com/en/upload/-img-lapin1.png)
Cat# 31-1013-00
Size : 100uL
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to β-actin (beta-actin)
100 µL
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