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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CBX5 antibody (92-191) (Clone: S4MR)

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Cat# NB-22-48363-100

Size : 100uL

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Phone : +1 850 650 7790

Brand : Neo Biotech

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Contact local distributor :

Phone : +1 850 650 7790

Western blot analysis of extracts of various cell lines, using HP1 alpha/CBX5rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using HP1 alpha/CBX5rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry analysis of HP1 alpha/CBX5 in paraffin-embedded human colon carcinoma tissue using HP1 alpha/CBX5 rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of HP1 alpha/CBX5 in paraffin-embedded human lung cancer tissue using HP1 alpha/CBX5 rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of HP1 alpha/CBX5 in paraffin-embedded human thyroid cancer tissue using HP1 alpha/CBX5 rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of HP1 alpha/CBX5 in paraffin-embedded mouse brain tissue using HP1 alpha/CBX5 rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of HP1 alpha/CBX5 in paraffin-embedded mouse kidney tissue using HP1 alpha/CBX5 rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of HP1 alpha/CBX5 in paraffin-embedded mouse lung tissue using HP1 alpha/CBX5 rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of HP1 alpha/CBX5 in paraffin-embedded mouse testis tissue using HP1 alpha/CBX5 rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of HP1 alpha/CBX5 in paraffin-embedded rat brain tissue using HP1 alpha/CBX5 rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunohistochemistry analysis of HP1 alpha/CBX5 in paraffin-embedded rat colon tissue using HP1 alpha/CBX5 rabbit monoclonal antibody at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
Immunofluorescence analysis of C6 cells using HP1 alpha/CBX5rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 Mu g extracts of MCF7 cells using 3 Mu g HP1 alpha/CBX5 antibody . Western blot was performed from the immunoprecipitate using HP1 alpha/CBX5 antibody at a dilution of 1:1000.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF/IP
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-HP1 alpha (92-191) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.

Clonality:	Monoclonal
Clone ID:	S4MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200 IP 1:500-1:1000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	CBX5
Gene ID:	23468
Uniprot ID:	CBX5_HUMAN
Immunogen Region:	92-191
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 92-191 of human HP1 alpha/CBX5 (P45973).
Immunogen Sequence:	SNFSNSADDIKSKKKREQSN DIARGFERGLEPEKIIGATD SCGDLMFLMKWKDTDEADLV LAKEANVKCPQIVIAFYEER LTWHAYPEDAENKEKETAKS

Description

Post Translational Modifications	Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. Ubiquitinated.
Function	Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Protein Name	Chromobox Protein Homolog 5 Antigen P25 Heterochromatin Protein 1 Homolog Alpha Hp1 Alpha
Database Links	Reactome: R-HSA-4551638 Reactome: R-HSA-8953750 Reactome: R-HSA-983231
Cellular Localisation	Nucleus Chromosome Centromere Colocalizes With Hnrnpu In The Nucleus Component Of Centromeric And Pericentromeric Heterochromatin Associates With Chromosomes During Mitosis Associates Specifically With Chromatin During Metaphase And Anaphase Localizes To Sites Of Dna Damage
Alternative Antibody Names	Anti-Chromobox Protein Homolog 5 antibody Anti-Antigen P25 antibody Anti-Heterochromatin Protein 1 Homolog Alpha antibody Anti-Hp1 Alpha antibody Anti-CBX5 antibody Anti-HP1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance