Anti-Flavocytochrome b558 (Human) mAb
Cat# D162-3
Size : 100μg
Brand : MBL International
Contact local distributor :
Phone : +1 850 650 7790
Please Note: MBL International will be shutting down its operations effective December 31, 2024. Distribution of MBL products in the United States will be transferred to Cosmo Bio US on January 1st while European Distributors will remain unchanged. For any US inquiries regarding orders or support during this transition, reach out to Cosmo Bio: https://www.cosmobiousa.com/. For Non-US inquiries reach out to MBL in Japan: https://www.mblbio.com/.
| Target | Flavocytochrome b |
|---|---|
| Product Type | Antibody |
| Application | FCM |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG1 |
| Immunogen | Human cytochrome b rich fraction |
| Host Species | Mouse |
| Species Reactivity | Human |
| Formulation | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Research Area | Cell Biology |
| Description/Background | The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2-), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2-. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections. |
| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at -20°C. |
| Protocols | FCM |
| Clone Number | 7D5 |
| Concentration | 1 mg/mL |
| Source | This antibody was purified from hybridoma (clone 7D5) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Balb/c mouse splenocyte immunized with the human cytochrome b rich fraction. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
D162-3
Antibody
100 µl