Anti-GFP (RABBIT) Antibody
Cat# 600-401-215
Size : 100ug
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for GFP Antibody
Product Details
Anti-GFP (RABBIT) Antibody - 600-401-215
rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Rabbit
Polyclonal
IgG
Target Details
GFP, eGFP, rGFP, RS-GFP, S65T-GFP, YFP
Recombinant Protein
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
P42212 - UniProtKB
Application Details
ELISA, WB
EM, FC, IF, IHC, IP, Microarray, Other, Purification, Multiplex - View References
Anti-GFP antibody is designed to detect GFP and its variants. GFP antibody has been tested by western blot and ELISA. This product can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Formulation
1.25 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store Anti-GFP Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. GFP antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Green Fluorescent Protein (GFP) is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP Antibody is ideal for Cell Biology, Neuroscience and Cancer research.
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IF, Confocal Microscopy PubMed
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IF, Confocal Microscopy PubMed
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WB, IB, PCA PubMed
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IP, Co-IP PubMed
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IF, Confocal Microscopy PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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IF, Confocal Microscopy; Multiplex Assay PubMed
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IF, Confocal Microscopy PubMed
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IF, Confocal Microscopy PubMed
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IF, Confocal Microscopy PubMed
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WB, IB, PCA PubMed
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IF, Confocal Microscopy; Multiplex Assay PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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IHC, ICC, Histology PubMed
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IHC, ICC, Histology PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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IF, Confocal Microscopy; Multiplex Assay PubMed
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Applications
WB, IB, PCA PubMed
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Applications
WB, IB, PCA PubMed
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Bead Conjugation PubMed
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Applications
IF, Confocal Microscopy PubMed
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IHC, ICC, Histology PubMed
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Ab Purification PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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Applications
Immuno-EM PubMed
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Applications
Microarray, Microarray chip PubMed
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Applications
IHC, ICC, Histology; Multiplex Assay PubMed
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Applications
IF, Confocal Microscopy; IP, Co-IP; WB, IB, PCA; Multiplex Assay PubMed
Oleinikov AV et al. (2009). High throughput functional assays of the variant antigen PfEMP1 reveal a single domain in the 3D7 Plasmodium falciparum genome that binds ICAM1 with high affinity and is targeted by naturally acquired neutralizing antibodies. PloS Pathogens
Applications
Ab Immobilization PubMed
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Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Flow Cytometry (FC) Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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